Department of Internal Medicine, Federal University of Paraná, Curitiba 80.060-240, Brazil.
Department of Pathology, Federal University of Paraná, PR, Curitiba 80.060-240, Brazil.
Int J Mol Sci. 2018 Dec 22;20(1):46. doi: 10.3390/ijms20010046.
The genus includes several strains isolated from different grasses. The identification of non-coding RNAs (ncRNAs) in the genus is an important stage studying the interaction of these molecules and the way they modulate physiological responses of different mechanisms, through RNA⁻RNA interaction or RNA⁻protein interaction. This interaction with their target occurs through the perfect pairing of short sequences (-encoded ncRNAs) or by the partial pairing of short sequences (-encoded ncRNAs). However, the companion Hfq can stabilize interactions in the -acting class. In addition, there are Riboswitches, located at the 5' end of mRNA and less often at the 3' end, which respond to environmental signals, high temperatures, or small binder molecules. Recently, CRISPR (clustered regularly interspaced palindromic repeats), in prokaryotes, have been described that consist of serial repeats of base sequences (spacer DNA) resulting from a previous exposure to exogenous plasmids or bacteriophages. We identified 285 ncRNAs in () SmR1, expressed in different experimental conditions of RNA-seq material, classified as -encoded ncRNAs or -encoded ncRNAs and detected RNA domains and CRISPR sequences. The results provide a better understanding of the participation of this type of RNA in the regulation of the metabolism of bacteria of the genus spp.
该属包括从不同草种中分离出的多个菌株。研究这些分子的相互作用以及它们通过 RNA-RNA 相互作用或 RNA-蛋白质相互作用调节不同机制生理反应的方式,鉴定该属中的非编码 RNA(ncRNA)是一个重要阶段。这种与靶标的相互作用通过短序列(-编码 ncRNA)的完全配对或短序列(-编码 ncRNA)的部分配对发生。然而,伴侣蛋白 Hfq 可以稳定 -作用类的相互作用。此外,还有位于 mRNA 5'端且较少位于 3'端的核糖开关,它们可以响应环境信号、高温或小分子结合物。最近,在原核生物中描述了 CRISPR(成簇规律间隔短回文重复序列),它由先前暴露于外源性质粒或噬菌体的碱基序列(间隔 DNA)的串联重复组成。我们在 () SmR1 中鉴定了 285 个 ncRNA,这些 ncRNA 在 RNA-seq 材料的不同实验条件下表达,分为-编码 ncRNA 或-编码 ncRNA,并检测到 RNA 结构域和 CRISPR 序列。这些结果有助于更好地理解这种类型的 RNA 在调节属 spp 细菌代谢中的作用。