Szychowski Konrad A, Binduga Urszula E, Rybczyńska-Tkaczyk Kamila, Leja Marcin L, Gmiński Jan
Department of Public Health, Dietetics and Lifestyle Disorders, Faculty of Medicine, University of Information Technology and Management in Rzeszow, Sucharskiego 2, Rzeszow 35-225, Poland.
Department of Environmental Microbiology, Laboratory of Mycology, University of Life Sciences, Leszczyńskiego 7, Lublin 20-069, Poland.
Saudi J Biol Sci. 2018 Dec;25(8):1703-1712. doi: 10.1016/j.sjbs.2016.10.005. Epub 2016 Oct 12.
Garlic ( L., Alliaceae) has acquired a reputation as a therapeutic agent and herbal remedy to prevent and treat several pathologies. The aim of the present study was to determine the effect of two L. cultivars, Harnaś and Morado, on reactive oxygen species (ROS) production, viability and apoptotic process in human squamous carcinoma cell line SCC-15. The experiments were conducted on SCC-15 cell line exposed to increasing concentrations of garlic extracts of 0.062, 0.125, 0.250, 0.500 and 1.000 mg/mL. After the experiments, ROS formation, caspase-3 activity and neutral red uptake were measured in the cells, and in a collected medium lactate dehydrogenase (LDH) release was measured. The Spanish cultivar Morado has demonstrated higher potential to stimulate ROS production in SCC-15 cells after a short time period (6 h) than the Polish cultivar Harnaś. However, the Polish cultivar Harnaś manifested more prolonged potential to stimulate ROS production in SCC-15 cells. Both studied garlic extracts induced cytotoxicity on SCC-15 cell line which was probably ROS-dependent. We also determined that in SCC-15 cells high concentrations of studied extracts did not cause activation of caspase-3 which suggested caspase-independent or necrotic cell death.
大蒜（L.，葱科）作为一种预防和治疗多种疾病的治疗剂和草药已声名远扬。本研究的目的是确定两种大蒜品种，即哈纳斯（Harnaś）和莫拉多（Morado），对人鳞状癌细胞系SCC - 15中活性氧（ROS）产生、细胞活力和凋亡过程的影响。实验在暴露于浓度递增的大蒜提取物（0.062、0.125、0.250、0.500和1.000 mg/mL）的SCC - 15细胞系上进行。实验后，测量细胞中的ROS形成、半胱天冬酶 - 3活性和中性红摄取，并在收集的培养基中测量乳酸脱氢酶（LDH）释放。西班牙品种莫拉多在短时间（6小时）后比波兰品种哈纳斯表现出更高的刺激SCC - 15细胞中ROS产生的潜力。然而，波兰品种哈纳斯在刺激SCC - 15细胞中ROS产生方面表现出更持久的潜力。两种研究的大蒜提取物均对SCC - 15细胞系诱导了细胞毒性，这可能是ROS依赖性的。我们还确定，在SCC - 15细胞中，高浓度的研究提取物并未导致半胱天冬酶 - 3的激活，这表明细胞死亡是不依赖半胱天冬酶的或坏死性的。
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