地中海甜柠檬（Citrus limetta Risso）汁的类黄酮谱及自由基清除活性
Flavonoid profile and radical-scavenging activity of Mediterranean sweet lemon (Citrus limetta Risso) juice.
作者信息
Barreca Davide, Bellocco Ersilia, Caristi Corrado, Leuzzi Ugo, Gattuso Giuseppe
机构信息
Dipartimento di Chimica Organica e Biologica, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.
Dipartimento di Chimica Organica e Biologica, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.
出版信息
Food Chem. 2011 Nov 15;129(2):417-422. doi: 10.1016/j.foodchem.2011.04.093. Epub 2011 May 4.
The flavonoid composition of crude Citrus limetta Risso (limetta, Mediterranean sweet lemon) juice was elucidated by means of reverse-phase HPLC-DAD-ESI-MS/MS analysis. In a single course, eight compounds (C- and O-glycosyl flavonoids) were identified, six of which were found for the first time in limetta juice: four C-glucosyl flavones, namely vicenin-2, lucenin-2 4'-methyl ether, orientin 4'-methyl ether and scoparin; the O-glycosyl flavone, rhoifolin; and the O-glycosyl flavanone, eriocitrin. In addition, ABTS and DPPH radical quenching and FRAP assays demonstrate that limetta juice possesses remarkable antioxidant activity.
通过反相高效液相色谱-二极管阵列检测器-电喷雾串联质谱（HPLC-DAD-ESI-MS/MS）分析，阐明了粗制地中海甜柠檬汁（地中海甜柠檬）的类黄酮成分。在一次分析过程中，鉴定出了8种化合物（C-和O-糖基黄酮类化合物），其中6种是首次在地中海甜柠檬果汁中发现：4种C-葡萄糖基黄酮，即异荭草苷、异槲皮苷4'-甲醚、东方草苷4'-甲醚和滨蓟黄苷；O-糖基黄酮，芸香柚皮苷；以及O-糖基黄烷酮，圣草次苷。此外，ABTS和DPPH自由基淬灭以及铁还原抗氧化能力（FRAP）测定表明，地中海甜柠檬果汁具有显著的抗氧化活性。
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