Evaluation of hyperbaric oxygen therapy for spinal cord injury in rats with different treatment course using diffusion tensor imaging.

STUDY DESIGN

Animal study.

OBJECTIVES

To evaluate the efficacy of hyperbaric oxygen (HBO) therapy for spinal cord injury (SCI) in rats with different treatment course using diffusion tensor imaging (DTI).

SETTING

Hospital in Fuzhou, China.

METHODS

Fifty adult Sprague-Dawley rats were grouped as: (A) sham-operated group (n = 10); (B) SCI without HBO therapy group (n = 10); (C) SCI with HBO therapy for 2 weeks (SCI+HBO) group (n = 10); (D) SCI with HBO therapy for 4 weeks (SCI+HBO) group (n = 10); (E) SCI with HBO therapy for 6 weeks (SCI+HBO) group (n = 10). Basso Beattie Bresnahan (BBB) scores and diffusion tensor imaging parameters including fractional anisotropy (FA), mean diffusivity (MD), radial diffusion (RD), and axial diffusion (AD) values in the injury epicenter, as well as 2 mm rostral and caudal to the injury epicenter were collected and analyzed 6 weeks post-injury.

RESULTS

Higher BBB score and FA values were found in the SCI+HBO group than in the SCI and SCI+HBO groups (all P < 0.05), whereas no significant differences of these metrics were observed between the SCI+HBO and SCI+HBO groups. MD and RD values of the SCI+HBO group were significantly lower than those of the SCI group (all P < 0.01). FA values were positively correlated with BBB scores. MD and RD values were negatively correlated with BBB scores.

CONCLUSION

DTI parameters, especially FA, could non-invasively and quantifiably evaluate the efficacy of HBO treatment for rats with SCI and 4 weeks may be the more appropriate treatment course.