一种用于检测 SO 衍生物的新型比色和比率荧光探针及其在活细胞中的生物成像。
A novel colorimetric and ratiometric fluorescent probe for sensing SO derivatives and their bio-imaging in living cells.
机构信息
Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, Zhejiang 315211, China.
出版信息
Analyst. 2019 Feb 25;144(5):1546-1554. doi: 10.1039/c8an02331j.
We report a novel fluorescent probe HBN-TCF for the detection of SO2 derivatives. This probe exhibited near-infrared fluorescence emission with an excitation wavelength of 620 nm. After reacting with SO32-, the emission channel at 664 nm decreased, while the new strong emission channel at 482 nm increased (λex = 400 nm), with a large emission distance (Δλ = 182 nm) observed. This probe exhibited the rapid and selective detection of SO2 derivatives compared with other sulfur-containing species and featured a low detection limit (82 nM). This colorimetric and ratiometric fluorescent probe showed high selectivity and sensitivity for detecting SO2 derivatives. The probe was also successfully exploited for the fluorescence imaging of intracellular and exogenous SO2 derivatives in BEL-7402 cells.
我们报告了一种用于检测 SO2 衍生物的新型荧光探针 HBN-TCF。该探针在 620nm 的激发波长下显示出近红外荧光发射。与 SO32-反应后，发射通道在 664nm 处降低，而在 482nm 处新的强发射通道增加（λex = 400nm），观察到大的发射距离（Δλ = 182nm）。与其他含硫物种相比，该探针表现出对 SO2 衍生物的快速和选择性检测，并且具有低的检测限（82 nM）。这种比色和比率荧光探针对检测 SO2 衍生物表现出高的选择性和灵敏度。该探针还成功地用于 BEL-7402 细胞中内源性和外源性 SO2 衍生物的荧光成像。
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