Bo Wang, Yan Ying, Xu Jing, Fu Xiaoyan, Han Hongjuan, Gao Jianjie, Li Zhenjun, Wang Lijuan, Tian Yongsheng, Peng Rihe, Yao Quanhong
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, P.R. China.
Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, P.R. China.
J Microbiol Biotechnol. 2018 Dec 28;28(12):2057-2063. doi: 10.4014/jmb.1807.08042.
Laccases can oxidize a variety of phenolic and non-phenolic substrates including synthetic dyes. In this research, a laccase gene from was chemically synthesized and optimized to heterogeneous expression in and . The properties of recombinant laccase expressed by were investigated. The laccase activity was optimal at 3.6 pH and 40°C. It exhibited and values of 0.565 mmol l⁻¹ and 1.51 μmol l⁻¹ min⁻¹ for ABTS respectively. As compared with untransformed control plants, the laccase activity in crude extracts of transgenic lines exhibited a 5.4 to 12.4-fold increase. Both laccases expressed in transgenic or could decolorize crystal violet. These results indicated that laccase gene may be transgenically exploited in fungi or plants for dye decolorization.
漆酶能够氧化多种酚类和非酚类底物，包括合成染料。在本研究中，一个来自[具体来源未给出]的漆酶基因被化学合成并优化，以便在[具体物种1]和[具体物种2]中进行异源表达。对[具体物种]表达的重组漆酶的特性进行了研究。漆酶活性在pH 3.6和40°C时最佳。其对ABTS的Km和Vmax值分别为0.565 mmol l⁻¹和1.51 μmol l⁻¹ min⁻¹。与未转化的对照植物相比，转基因株系粗提物中的漆酶活性提高了5.4至12.4倍。在转基因[具体物种1]或[具体物种2]中表达的两种漆酶都能使结晶紫脱色。这些结果表明，[具体来源]漆酶基因可通过转基因方式在真菌或植物中用于染料脱色。
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