一种从人牙髓干细胞中诱导生成成熟少突胶质细胞的高效诱导方案。
An efficient induction protocol for deriving mature oligodendrocytes from human dental stem cells.
作者信息
Bagheri-Hosseinabadi Z, Hamidabadi H Ghasemi, Bojnordi M Nazm
出版信息
Bratisl Lek Listy. 2019;120(1):86-88. doi: 10.4149/BLL_2019_013.
INTRODUCTION
This study examined the process of deriving mature oligodendrocytes from human dental pulp stem cells (hDPSCs) via addition of cerebrospinal fluid (CSF).
METHODS
The hDPSCs were cultured in the presence of retinoic acid and CSF. The oligodendrocytes were confirmed using immunocytochemistry for specific glial markers, namely Olig2 and MBP markers.
RESULTS
The differentiated oligodendrocytes were immunopositive for Olig2 and MBP markers at the end of induction phase.
CONCLUSION
It is concluded that this study indicated the glial differentiation of hDPSCs in the presence of CSF and appropriate inducers, which is a usable therapeutic technique in neuroregenerative medicine (Fig. 3, Ref. 24).
引言
本研究通过添加脑脊液（CSF）研究了从人牙髓干细胞（hDPSCs）中获得成熟少突胶质细胞的过程。
方法
将hDPSCs在视黄酸和CSF存在的条件下进行培养。使用针对特定神经胶质标志物（即少突胶质细胞转录因子2（Olig2）和髓鞘碱性蛋白（MBP）标志物）的免疫细胞化学方法来确认少突胶质细胞。
结果
在诱导阶段结束时，分化的少突胶质细胞对Olig2和MBP标志物呈免疫阳性。
结论
得出的结论是，本研究表明在CSF和适当诱导剂存在的情况下，hDPSCs可发生神经胶质分化，这是神经再生医学中一种可用的治疗技术（图3，参考文献24）。
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