长链非编码RNA FENDRR促进高糖诱导的人视网膜内皮细胞增殖和血管生成。
LncRNA FENDRR promotes high-glucose-induced proliferation and angiogenesis of human retinal endothelial cells.
作者信息
Shi Yu, Chen Chunsheng, Xu Yidan, Liu Yi, Zhang Hui, Liu Yang
机构信息
a Department of Ophthalmology, Nanjing First Hospital , Nanjing Medical University , Nanjing , Jiangsu Province , China.
b Department of Ophthalmology, Sir Run Run Hospital , Nanjing Medical University , Nanjing , Jiangsu Province , China.
出版信息
Biosci Biotechnol Biochem. 2019 May;83(5):869-875. doi: 10.1080/09168451.2019.1569499. Epub 2019 Jan 30.
The study aimed to investigate the role of lncRNA FENDRR in proliferation and angiogenesis of human retinal endothelial cells (HRECs). HRECs were cultured in high-glucose medium to mimic diabetic retinopathy (DR) model. We overexpressed or knocked down FENDRR in HRECs to evaluate the effect of FENDRR expression on cell proliferation, migration, and capillary morphogenesis of HRECs under either normal glucose or high glucose condition. Results showed that VEGF and FENDRR expression were increased in blood from DR patients compared with the control subjects. Furthermore, high glucose treatment upregulated expression of VEGF and FENDRR secreted from HRECs, in a dose- and time-dependent manner. Importantly, FENDRR overexpression significantly promoted the high-glucose-induced proliferation, migration, capillary morphogenesis, and VEGF expression in HRECs. In contrast, FENDRR knockdown exerted the opposite effects. In conclusion, lncRNA FENDRR promotes the high-glucose-induced proliferation and angiogenesis of HRECs and may serve as a potential target for anti-angiogenic therapy for DR.
该研究旨在探讨长链非编码RNA FENDRR在人视网膜内皮细胞（HRECs）增殖和血管生成中的作用。将HRECs培养于高糖培养基中以模拟糖尿病视网膜病变（DR）模型。我们在HRECs中过表达或敲低FENDRR，以评估在正常葡萄糖或高糖条件下FENDRR表达对HRECs细胞增殖、迁移和毛细血管形态发生的影响。结果显示，与对照组相比，DR患者血液中VEGF和FENDRR表达增加。此外，高糖处理以剂量和时间依赖性方式上调HRECs分泌的VEGF和FENDRR的表达。重要的是，FENDRR过表达显著促进高糖诱导的HRECs增殖、迁移、毛细血管形态发生及VEGF表达。相反，FENDRR敲低则产生相反的效果。总之，长链非编码RNA FENDRR促进高糖诱导的HRECs增殖和血管生成，可能成为DR抗血管生成治疗的潜在靶点。
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