膳食壳聚糖-硒纳米颗粒（CTS-SeNP）增强斑马鱼的免疫力和抗病能力。
Dietary chitosan-selenium nanoparticle (CTS-SeNP) enhance immunity and disease resistance in zebrafish.
机构信息
The Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen, PR China; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China.
Key Laboratory of South China Sea Fisheries Resources Exploitation & Utilization, Ministry of Agriculture, Guangzhou, China.
出版信息
Fish Shellfish Immunol. 2019 Apr;87:449-459. doi: 10.1016/j.fsi.2019.01.042. Epub 2019 Jan 28.
Selenium (Se) is an essential micronutrient for human and animals. It plays an important role in antioxidative stress, selenoenzymes regulation and immunomodulation. In this study, two common immunostimulants chitosan (CTS) and Se were used to synthesize nanoparticles (CTS-SeNP). Immunomodulation of CTS-SeNP were explored in wild-type zebrafish (Danio rerio). Dietary supplementation of CTS-SeNP enhanced lysozyme activity, phagocytic respiratory burst as well as splenocytes proliferation stimulated by LPS and ConA. CTS-SeNP showed immunomodulation effect from 5 to 20 μg/g but the best outcome was observed at 10 μg/g. Immunomodulation effect were rapidly induced after 3-9d and can sustain to 60. The zebrafish fed with 10 μg/g CTS-SeNP also showed 26.7% higher survival rate than the control after intraperitoneal injection of common bacterium Aeromonas hydrophila. Our results suggested that CTS-SeNP is an effective immunostimulant to fish and has potential application in aquaculture.
硒（Se）是人类和动物必需的微量元素。它在抗氧化应激、硒酶调节和免疫调节中发挥着重要作用。在这项研究中，两种常用的免疫刺激剂壳聚糖（CTS）和 Se 被用于合成纳米颗粒（CTS-SeNP）。在野生型斑马鱼（Danio rerio）中探索了 CTS-SeNP 的免疫调节作用。膳食补充 CTS-SeNP 可增强溶菌酶活性、吞噬细胞呼吸爆发以及 LPS 和 ConA 刺激的脾细胞增殖。CTS-SeNP 从 5 至 20μg/g 表现出免疫调节作用，但在 10μg/g 时观察到最佳效果。免疫调节作用在 3-9 天后迅速诱导，并可持续至 60 天。与对照组相比，经腹腔注射普通细菌嗜水气单胞菌后，摄食 10μg/g CTS-SeNP 的斑马鱼的存活率也提高了 26.7%。我们的结果表明，CTS-SeNP 是一种有效的鱼类免疫刺激剂，在水产养殖中有潜在的应用前景。
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