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从樱桃李果实中简便合成荧光碳点用于荧光墨水、Fe 离子检测和细胞成像。

Facile synthesis of fluorescent carbon dots from Prunus cerasifera fruits for fluorescent ink, Fe ion detection and cell imaging.

机构信息

College of Pharmacy, Dalian Medical University, Dalian 116044, People's Republic of China; College of Medical Laboratory, Dalian Medical University, Dalian 116044, People's Republic of China.

College of Pharmacy, Dalian Medical University, Dalian 116044, People's Republic of China.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2019 Apr 15;213:281-287. doi: 10.1016/j.saa.2019.01.079. Epub 2019 Jan 28.

Abstract

Carbon dots (CDs) synthesized from natural products have drawn numerous attentions due to some unique properties. Here, Prunus cerasifera fruits were used as carbon source to synthesize high luminescent CDs by hydrothermal method. The obtained CDs were characterized by TEM, FTIR and XPS methods, founding the CDs were near-spherical and contained abundant nitrogen element. The CDs aqueous solution exhibited bright blue fluorescence under ultraviolet illumination, with the maximum emission at 450 nm. They could be potentially used as invisible fluorescent ink by written on the paper and irradiated by UV light, due to their fluorescent properties. Moreover, the CDs were found being selectively quenched by Fe ion. The quench of CDs was linearly related to the concentration of Fe ion in the range of 0-0.5 mM, meaning they could be developed as fluorescent probe of Fe ion. At last, the CDs were used for cell imaging, founding they were low toxicity to HepG2 cells and exhibited blue and green fluorescence under a fluorescence microscope. In summary, the CDs prepared from Prunus cerasifera fruits exhibited excellent fluorescence properties, and could be potentially applied in the field of fluorescent ink, Fe ion detection and cell imaging.

摘要

碳点(CDs)由天然产物合成,由于具有一些独特的性质而引起了广泛关注。 在这里,我们使用樱桃李果实作为碳源,通过水热法合成了具有高光致发光性能的 CDs。 通过 TEM、FTIR 和 XPS 方法对所得到的 CDs 进行了表征,发现 CDs 为近球形且含有丰富的氮元素。 CDs 水溶液在紫外光照射下发出明亮的蓝色荧光,最大发射波长为 450nm。 由于其荧光特性,它们可以用作隐形荧光墨水,在纸上书写并通过紫外光照射即可显现。 此外,我们发现 CDs 可以被 Fe 离子选择性猝灭。 CDs 的猝灭与 Fe 离子浓度在 0-0.5mM 范围内呈线性关系,这意味着它们可以被开发为 Fe 离子的荧光探针。 最后,我们将 CDs 用于细胞成像,发现它们对 HepG2 细胞的毒性较低,在荧光显微镜下发出蓝色和绿色荧光。 综上所述,由樱桃李果实制备的 CDs 表现出优异的荧光性能,有望应用于荧光墨水、Fe 离子检测和细胞成像领域。

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