纳米管辅助微波电穿孔用于单细胞病原体鉴定和抗菌药物敏感性测试。
Nanotube assisted microwave electroporation for single cell pathogen identification and antimicrobial susceptibility testing.
机构信息
Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA, USA.
Department of Mechanical Engineering, The Pennsylvania State University, University Park, PA, USA.
出版信息
Nanomedicine. 2019 Apr;17:246-253. doi: 10.1016/j.nano.2019.01.015. Epub 2019 Feb 20.
A nanotube assisted microwave electroporation (NAME) technique is demonstrated for delivering molecular biosensors into viable bacteria for multiplex single cell pathogen identification to advance rapid diagnostics in clinical microbiology. Due to the small volume of a bacterial cell (~femtoliter), the intracellular concentration of the target molecule is high, which results in a strong signal for single cell detection without amplification. The NAME procedure can be completed in as little as 30 minutes and can achieve over 90% transformation efficiency. We demonstrate the feasibility of NAME for identifying clinical isolates of bloodborne and uropathogenic pathogens and detecting bacterial pathogens directly from patient's samples. In conjunction with a microfluidic single cell trapping technique, NAME allows single cell pathogen identification and antimicrobial susceptibility testing concurrently. Using this approach, the time for microbiological analysis reduces from days to hours, which will have a significant impact on the clinical management of bacterial infections.
一种基于纳米管的微波电穿孔（NAME）技术被用于将分子生物传感器递送至活细菌中，以实现多重单细胞病原体识别，从而推进临床微生物学的快速诊断。由于细菌细胞的体积非常小（约为飞升），目标分子的细胞内浓度很高，因此无需扩增即可实现单细胞检测的强信号。NAME 过程可以在 30 分钟内完成，并且可以实现超过 90%的转化效率。我们证明了 NAME 用于鉴定血液和尿路感染病原体的临床分离株以及直接从患者样本中检测细菌病原体的可行性。结合微流控单细胞捕获技术，NAME 允许同时进行单细胞病原体识别和抗菌药物敏感性测试。使用这种方法，微生物分析所需的时间从数天缩短到数小时，这将对细菌感染的临床管理产生重大影响。
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