Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Bioorg Med Chem. 2019 Apr 1;27(7):1444-1448. doi: 10.1016/j.bmc.2019.02.036. Epub 2019 Feb 18.
We designed a conjugated molecule bearing an O-nitrobenzoxadiazole (O-NBD) unit and an acetylated trimethyl lock as a chromogenic and fluorogenic probe for the detection of esterase activity. The designed molecule was briefly synthesized from a commercially available compound in two steps. Several experiments revealed that the conjugated molecule serves as a sensitive chromogenic and fluorogenic probe for the detection of porcine liver esterase activity. Mechanistic studies indicated that an intramolecular O- to N-NBD migration is involved in the chromogenic/fluorogenic phenomena. The results here would be helpful for designing other O-NBD-based chromogenic/fluorogenic probes in future.
我们设计了一种带有邻硝基苯并二唑（O-NBD）单元和乙酰化三甲基锁的共轭分子，作为检测酯酶活性的显色和荧光探针。设计的分子通过两步从商业可得的化合物中简要合成。多项实验表明，该共轭分子可作为一种灵敏的显色和荧光探针，用于检测猪肝酯酶活性。机理研究表明，分子内 O 到 N-NBD 的迁移参与了显色/荧光现象。本研究结果将有助于未来设计其他基于 O-NBD 的显色/荧光探针。
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