两种水硬性硅酸钙基水泥的细胞相互作用及抗菌效果：细胞依赖模型
Cellular interaction and antibacterial efficacy of two hydraulic calcium silicate-based cements: Cell-dependent model.
作者信息
Fathy Salma M, Abd El-Aziz Abeer M, Labah Doaa A
机构信息
Department of Dental Biomaterials, Faculty of Oral and Dental Surgery, Zagazig University, Zagazig, Egypt.
Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
出版信息
J Conserv Dent. 2019 Jan-Feb;22(1):17-22. doi: 10.4103/JCD.JCD_272_18.
BACKGROUND
This study investigated cytotoxic probability, osteogenic potential, and antibacterial efficacy of two pulp-capping hydraulic calcium-silicate cements.
MATERIALS AND METHODS
For osteogenic potential and cytotoxicity evaluation, mesenchymal stem cells (MSCs) and materials disc-shaped specimens were used. Increase or decrease in a number of proliferating MSCs was calculated after three intervals. Alkaline phosphatase (ALP) levels in osteogenic media were normalized to the total protein content of cells and measured spectrophotometrically. Antibacterial efficiency through growth curves of in direct contact with tested materials.
RESULTS
Biodentine showed the highest number of proliferating MSCs (278000.41 ± 4000.06, after 72 h) and the highest concentration of ALP after 12 days (209.26 ± 7.17 μU/μg protein). It showed the lowest slope (0.003 ± 0.0005) of strains growth curves after 18 h.
CONCLUSION
Biodentine proved a highly significant osteogenic ability and gave a significant reduction of growth.
背景
本研究调查了两种用于盖髓的水硬性硅酸钙水泥的细胞毒性概率、成骨潜力和抗菌功效。
材料与方法
为评估成骨潜力和细胞毒性，使用了间充质干细胞（MSCs）和材料圆盘状标本。在三个时间间隔后计算增殖的间充质干细胞数量的增加或减少。将成骨培养基中的碱性磷酸酶（ALP）水平标准化为细胞的总蛋白含量，并通过分光光度法进行测量。通过与测试材料直接接触的细菌生长曲线评估抗菌效率。
结果
BioRoot RCS在72小时后显示出最高的增殖间充质干细胞数量（278000.41±4000.06），在12天后显示出最高的碱性磷酸酶浓度（209.26±7.17μU/μg蛋白）。它在18小时后显示出细菌菌株生长曲线的最低斜率（0.003±0.0005）。
结论
BioRoot RCS证明具有高度显著的成骨能力，并显著降低了细菌生长。 （注：原文中“RESULTS”部分开头的“Biodentine”应为“BioRoot RCS”，译文已修正）
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