番茄斑萎病毒在伊朗马铃薯上的首次报道。

First Report of Tomato Spotted Wilt Virus on Potatoes in Iran.

作者信息

Pourrahim R, Farzadfar Sh, Moini A A, Shahraeen N, Ahoonmanesh A

机构信息

Plant Virology Department, Plant Pests and Diseases Research Institute, P.O.Box 19395-1454, Tehran, Iran.

Plant Protection Department, Isfahan University of Technology, Isfahan, Iran.

出版信息

Plant Dis. 2001 Apr;85(4):442. doi: 10.1094/PDIS.2001.85.4.442B.

DOI:10.1094/PDIS.2001.85.4.442B

PMID:30831980

Abstract

Severe leaf and stem necrosis before flowering was observed in potato (Solanum tuberosum) fields of Firouzkoh Province, Iran, during the summer of 1998. Infected plants died before the end of the growing season. Necrosis was more severe in cv. Agria than in cvs. Ajaxs and Arinda. A high population of Thrips tabaci was observed in August and September. Tomato spotted wilt virus (TSWV) (1) was detected in affected potatoes by using specific TSWV-IgG (from Bioreba) in double-antibody sandwich enzyme linked immunosorbent assay and by indicator plant reactions. Mechanical inoculation of indicator plants with leaf extracts of symptomatic potatoes produce necrotic local lesions in Chenopodium quinoa, C. amaranticolor, Gomphrena globosa, Vicia faba, Vigna sinensis, Phaseolus aureus var. Gohar, P. vulgaris, and Petunia hybrida. The virus caused systemic necrosis in Capsicum frutescens, Datura stramonium, D. metel, Nicotiana glutinosa, N. rustica, and Trapaeolum majus, preceded by systemic chlorotic spots. TSWV was reported from ornamental crops in Tehran and Absard areas near to Firouzkoh province (2), but this is the first report of TSWV occurrence on potatoes in Iran. References: (1) T. S. Ie. Descriptions of Plant Viruses. No. 39, 1970. (2) A. A. Moeini, et al. Iran. J. Plant Pathol. (In press.).

摘要

1998年夏季，在伊朗菲鲁兹科省的马铃薯（Solanum tuberosum）田块中，观察到开花前叶片和茎严重坏死。受感染植株在生长季结束前死亡。坏死在Agria品种中比Ajaxs和Arinda品种中更严重。8月和9月观察到大量烟蓟马。通过双抗体夹心酶联免疫吸附测定法使用特异性番茄斑萎病毒免疫球蛋白（来自Bioreba公司）以及通过指示植物反应，在受影响的马铃薯中检测到番茄斑萎病毒（TSWV）。用有症状马铃薯的叶片提取物对指示植物进行机械接种，在藜麦、苋色藜、千日红、蚕豆、豇豆、金甲绿豆、菜豆、矮牵牛中产生坏死局部病斑。该病毒在辣椒、曼陀罗、毛曼陀罗、粘烟草、黄花烟草、旱金莲中引起系统坏死，之前有系统褪绿斑点。在德黑兰和靠近菲鲁兹科省的阿萨尔地区的观赏作物中曾报道过番茄斑萎病毒（2），但这是伊朗马铃薯上发生番茄斑萎病毒的首次报道。参考文献：（1）T. S. Ie. 《植物病毒描述》。第39号，1970年。（2）A. A. Moeini等人。《伊朗植物病理学杂志》（即将出版）。