从血浆衍生的治疗药物中灭活和去除基孔肯雅病毒和马亚罗病毒。
Inactivation and Removal of Chikungunya Virus and Mayaro Virus from Plasma-derived Medicinal Products.
机构信息
Department of Virology, Paul-Ehrlich-Institut, 63225 Langen, Germany.
Asahi Kasei, 51105 Cologne, Germany.
出版信息
Viruses. 2019 Mar 7;11(3):234. doi: 10.3390/v11030234.
BACKGROUND
Chikungunya virus (CHIKV) and Mayaro virus (MAYV) are closely related members of the Semliki Forest complex within the genus and are transmitted by arthropods, causing acute febrile illness in humans. CHIKV has spread to almost all continents, whereas autochthonous MAYV infections have been reported in South America and in the Caribbean. Nevertheless, there was concern about potential spread of MAYV to other regions similar to CHIKV in the past. The risk for transmission of emerging viruses by blood transfusion and the safety of plasma-derived medicinal products (PDMPs) are constant concerns. The manufacturing processes of PDMPs include procedures to inactivate/remove viruses.
METHODS
In this study, we investigated the reduction of MAYV and CHIKV by heat inactivation in various matrices, solvent/detergent treatment and nanofiltration.
RESULTS
Unexpectedly, MAYV was significantly more resistant to heat and solvent/detergent treatment compared to CHIKV. However, being similar in size, both MAYV and CHIKV were removed below the detection limit by 35 nm virus filters.
CONCLUSIONS
The inactivation profiles of different members vary considerably, even within the Semliki Forest Complex. However, robust dedicated viral inactivation/removal procedures commonly used in the plasma product industry are effective in inactivating or removing MAYV and CHIKV.
背景
基孔肯雅病毒（CHIKV）和马亚罗病毒（MAYV）是辛德毕斯病毒属内塞姆利基森林病毒复合体的密切相关成员，通过节肢动物传播，导致人类急性发热疾病。CHIKV 已传播到几乎所有大陆，而本土 MAYV 感染已在南美洲和加勒比地区报告。然而，过去人们担心 MAYV 可能会像 CHIKV 一样传播到其他类似地区。通过输血传播新兴病毒的风险以及血浆衍生药物产品（PDMP）的安全性一直是人们关注的问题。PDMP 的制造工艺包括灭活/去除病毒的程序。
方法
在这项研究中，我们研究了不同基质中热失活、溶剂/去污剂处理和纳米过滤对 MAYV 和 CHIKV 的减少作用。
结果
出乎意料的是，与 CHIKV 相比，MAYV 对热和溶剂/去污剂处理的抵抗力明显更强。然而，由于大小相似，35nm 病毒过滤器可将 MAYV 和 CHIKV 均去除至检测限以下。
结论
不同 成员的失活动力学差异很大，即使在辛德毕斯森林病毒复合体中也是如此。然而，血浆产品行业中常用的稳健专用病毒灭活/去除程序可有效灭活或去除 MAYV 和 CHIKV。
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