中药方剂养肝解毒散结方抑制 TGF-β1 诱导的人肝癌 Bel-7402 细胞上皮间质转化及转移潜能。
Traditional Chinese medicine formulation Yanggan Jiedu Sanjie inhibits TGF-β1-induced epithelial-mesenchymal transition and metastatic potential in human hepatocarcinoma Bel-7402 cells.
机构信息
Institute of Traditional Chinese Medicine in Oncology, Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, People's Republic of China.
Department of Science & Technology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, People's Republic of China.
出版信息
BMC Complement Altern Med. 2019 Mar 15;19(1):67. doi: 10.1186/s12906-019-2477-9.
BACKGROUND
Epithelial-mesenchymal transition (EMT) is a vital process in cancer progression and metastasis. Yanggan Jiedu Sanjie (YGJDSJ) is Traditional Chinese Medicine formulation for liver cancer treatment. In the present study, we evaluated the effects of YGJDSJ on TGF-β1-induced EMT in hepatocellular carcinoma Bel-7402 cells.
METHODS
Bel-7402 cells were treated with TGF-β1 and YGJDSJ. EMT was identified by morphological changes and expression of marker proteins. Cell morphology was observed under a microscope. Protein expression and phosphorylation was detected by western blotting. Cell migration was measured by the scratch assay. Cell adhesion and invasion was detected by a commercial kit.
RESULTS
YGJDSJ reversed TGF-β1-induced morphological changes, as well as the expression of the EMT markers E-cadherin and N-cadherin in Bel-7402 cells. YGJDSJ also inhibited TGF-β1 up-regulated Smad3 phosphorylation and Snail expression in Bel-7402 cells. Moreover, YGJDSJ inhibited TGF-β1-induced cell adhesion, migration and invasion in Bel-7402 cells.
CONCLUSIONS
YGJDSJ inhibited TGF-β1-induced EMT and mediated metastatic potential of Bel-7402 cells, which may be related to down-regulation of Smad3 phosphorylation and Snail expression. The present study provides a new basis for application of this herbal formula for prevention of liver cancer metastasis.
背景
上皮间质转化（EMT）是癌症进展和转移的重要过程。养肝解毒散结（YGJDSJ）是一种用于治疗肝癌的中药方剂。在本研究中，我们评估了 YGJDSJ 对 TGF-β1 诱导的肝癌 Bel-7402 细胞 EMT 的影响。
方法
用 TGF-β1 和 YGJDSJ 处理 Bel-7402 细胞。通过形态变化和标记蛋白的表达来鉴定 EMT。在显微镜下观察细胞形态。通过 Western blot 检测蛋白表达和磷酸化。通过划痕实验测量细胞迁移。通过商业试剂盒检测细胞黏附和侵袭。
结果
YGJDSJ 逆转了 TGF-β1 诱导的 Bel-7402 细胞形态变化以及 EMT 标志物 E-钙黏蛋白和 N-钙黏蛋白的表达。YGJDSJ 还抑制了 TGF-β1 上调的 Bel-7402 细胞中 Smad3 磷酸化和 Snail 的表达。此外，YGJDSJ 抑制了 TGF-β1 诱导的 Bel-7402 细胞黏附、迁移和侵袭。
结论
YGJDSJ 抑制 TGF-β1 诱导的 EMT，并介导 Bel-7402 细胞的转移潜能，这可能与下调 Smad3 磷酸化和 Snail 的表达有关。本研究为该中药方剂预防肝癌转移的应用提供了新的依据。
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