用于检测活细胞内源性和外源性硫化氢的比率型荧光探针。
A ratiometric fluorescent probe for detection of endogenous and exogenous hydrogen sulfide in living cells.
机构信息
Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu Province 730046, PR China.
出版信息
Talanta. 2019 Jun 1;198:185-192. doi: 10.1016/j.talanta.2019.02.017. Epub 2019 Feb 5.
A ratiometric visualized fluorescent probe of HS of intramolecular charge transfer (ICT) and excited intramolecular proton transfer (ESIPT) mechanisms due to solvation effects has been designed and synthesized. This chemosensor shows the distinct signal changes with dual-emission in blue and green fluorescence spectral channel (from 495 nm to 525 nm) upon the addition of HS in a single wavelength excitation. This chemosensor exhibits the low detection limit (91 nM) and high sensitivity and selectivity. Based on this, this chemosensor was successfully used not only to monitor HS exogenously but also used to detect and image the endogenously generated HS in HeLa cells with excellent performance.
一种基于分子内电荷转移（ICT）和激发态分子内质子转移（ESIPT）机制的比率型可视化荧光探针由于溶剂化效应而被设计和合成。该化学传感器在单一波长激发下，加入 HS 后，在蓝色和绿色荧光光谱通道（从 495nm 到 525nm）中显示出明显的信号变化，具有双重发射。该化学传感器具有低检测限（91nM）、高灵敏度和选择性。基于这一点，该化学传感器不仅成功地用于监测外源性 HS，而且还用于检测和成像 HeLa 细胞中内源性产生的 HS，具有优异的性能。
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