School of Biomedical Engineering, University of Western Ontario, 1151 Richmond St., London, ON N6A 5B9, Canada.
Department of Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond St., London, ON N6A 5B9, Canada.
Biosensors (Basel). 2019 Mar 16;9(1):42. doi: 10.3390/bios9010042.
Bioluminescence resonance energy transfer (BRET) techniques offer a high degree of sensitivity, reliability and ease of use for their application to sensing biomolecules. BRET is a distance dependent, non-radiative energy transfer, which uses a bioluminescent protein to excite an acceptor through the resonance energy transfer. A BRET sensor can quickly detect the change of a target biomolecule quantitatively without an external electromagnetic field, e.g., UV light, which normally can damage tissue. Having been developed quite recently, this technique has evolved rapidly. Here, different bioluminescent proteins have been reviewed. In addition to a multitude of bioluminescent proteins, this manuscript focuses on the recent development of BRET sensors by utilizing quantum dots. The special size-dependent properties of quantum dots have made the BRET sensing technique attractive for the real-time monitoring of the changes of target molecules and bioimaging in vivo. This review offers a look into the basis of the technique, donor/acceptor pairs, experimental applications and prospects.
生物发光共振能量转移（BRET）技术为生物分子传感提供了高度的灵敏度、可靠性和易用性。BRET 是一种依赖于距离的非辐射能量转移，它使用生物发光蛋白通过共振能量转移来激发受体。BRET 传感器可以快速定量检测目标生物分子的变化，而无需外部电磁场，例如紫外线，紫外线通常会损坏组织。这项技术最近才得到发展，发展迅速。在这里，我们回顾了不同的生物发光蛋白。除了多种生物发光蛋白外，本文还重点介绍了利用量子点开发 BRET 传感器的最新进展。量子点的特殊尺寸依赖性特性使得 BRET 传感技术在实时监测靶分子的变化和体内生物成像方面具有吸引力。本文综述了该技术的基础、供体/受体对、实验应用和前景。
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