Liu Baoyan, Lu Yanqin, Wang Yong, Ge Luna, Zhai Naixiang, Han Jinxiang
School of Medicine and Life Science, Shandong Academy of Medical Sciences, University of Jinan, 18877, Jingshi Road, Jinan, 250012, Shandong, China.
Key Laboratory for Rare Disease Research of Shandong Province, Shandong Medical Biotechnological Center, Shandong Academy of Medical Sciences, 18877, Jingshi Road, Jinan, 250012, Shandong, China.
Cell Tissue Bank. 2019 Jun;20(2):173-182. doi: 10.1007/s10561-019-09751-0. Epub 2019 Mar 18.
Calvaria from neonatal mouse and rat is ideal resource for osteoblasts but can be easily contaminated by other cells such as fibroblasts. Here, we established a protocol for isolation and purification of primary osteoblast by enzyme sequential digestion and differential adhesion. In addition, we compared the phenotypic and functional traits of osteoblasts from mouse and rat which are commonly employed in studies. The method applied equally to rat and mouse in osteoblasts isolation and was corroborated its feasibility and validity. The results also provided us evidences for other experiments such as choosing a certain time point to give intervention and do the relevant tests.
新生小鼠和大鼠的颅骨是成骨细胞的理想来源，但很容易被其他细胞如成纤维细胞污染。在此，我们建立了一种通过酶序贯消化和差异黏附来分离和纯化原代成骨细胞的方法。此外，我们比较了研究中常用的小鼠和大鼠成骨细胞的表型和功能特性。该方法在成骨细胞分离中同样适用于大鼠和小鼠，并证实了其可行性和有效性。这些结果也为我们进行其他实验提供了依据，比如选择某个时间点进行干预并做相关检测。
