Qin Xinxian, Luo Xiaoshuang, Chen Ye, Han Jiahua, Zhang Ji, Zhang Kankan, Hu Deyu
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, China.
Biomed Chromatogr. 2019 Jul;33(7):e4537. doi: 10.1002/bmc.4537. Epub 2019 Apr 17.
A simple pretreatment method with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated to simultaneously determine dichlorvos and phoxim in tobacco and soil matrices. Satisfactory linearity (R ≥ 0.9991) of the method was obtained for both analytes. The limits of detection and limits of quantification for dichlorvos and phoxim in three matrices were 0.0015-0.006 and 0.005-0.02 mg/kg, respectively. Average recoveries were 78.24-92.21% for dichlorvos and 76.62-100.51% for phoxim in soil, green tobacco leaves and cured tobacco leaves. The intra- and inter-day relative standard deviations were <6%. The established method was successfully applied for the residual analysis of dichlorvos and phoxim in real soil and tobacco samples. The results indicated that the established method could be used to detect trace amounts of dichlorvos and phoxim in tobacco. The data could also help the Chinese government establish maximum residue limits of dichlorvos and phoxim on tobacco and establish proper and safe use of dichlorvos and phoxim on tobacco plants in China.
开发并验证了一种采用液相色谱 - 串联质谱法（LC-MS/MS）的简单预处理方法，用于同时测定烟草和土壤基质中的敌敌畏和辛硫磷。两种分析物的方法线性良好（R≥0.9991）。三种基质中敌敌畏和辛硫磷的检测限和定量限分别为0.0015 - 0.006和0.005 - 0.02 mg/kg。土壤、鲜烟叶和烤烟叶中敌敌畏的平均回收率为78.24% - 92.21%，辛硫磷的平均回收率为76.62% - 100.51%。日内和日间相对标准偏差均<6%。所建立的方法成功应用于实际土壤和烟草样品中敌敌畏和辛硫磷的残留分析。结果表明，所建立的方法可用于检测烟草中痕量的敌敌畏和辛硫磷。这些数据也有助于中国政府制定烟草中敌敌畏和辛硫磷的最大残留限量，并确定在中国烟草植株上合理安全使用敌敌畏和辛硫磷的方法。
