pH 响应性木质素基两性表面活性剂等电点对木质纤维素酶水解的影响。
Effect of the isoelectric point of pH-responsive lignin-based amphoteric surfactant on the enzymatic hydrolysis of lignocellulose.
机构信息
School of Chemistry and Chemical Engineering, Guangdong Provincial Engineering Research Center for Green Fine Chemicals, South China University of Technology, Guangzhou, China.
School of Chemistry and Chemical Engineering, Guangdong Provincial Engineering Research Center for Green Fine Chemicals, South China University of Technology, Guangzhou, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China.
出版信息
Bioresour Technol. 2019 Jul;283:112-119. doi: 10.1016/j.biortech.2019.03.026. Epub 2019 Mar 7.
The isoelectric point (pI) of lignin-based surfactant is an important factor in the enhancement on the enzymatic hydrolysis of lignocellulose. In this work, lignin carboxylate (LC) and quaternary ammonium lignin carboxylates (LCQ-x, x%: the mass ratio of quaternizing agent to enzymatic hydrolysis lignin) with different isoelectric points were synthesized. LC or LCQ-x with pI significantly lower or higher than 4.8 reduced the non-productive adsorption of cellulase on lignin, but for the significant inhibitory effect on cellulase activity, their enhancements on the enzymatic hydrolysis of lignocellulose were not remarkable. However, LCQ-x with pI around 4.8 preserved the cellulase activity, and significantly reduced the non-productive adsorption of cellulase, therefore remarkably enhanced the enzymatic hydrolysis. 2 g/L LC, LCQ-40 (pI = 5.0) and LCQ-100 (pI = 9.2) increased the enzymatic digestibility of pretreated eucalyptus from 35.2% to 53.4%, 95.3% and 60.4% respectively. In addition, for the excellent pH-response performance, LCQ could be efficiently recovered after enzymatic saccharification.
木质素基表面活性剂的等电点（pI）是增强木质纤维素酶水解的一个重要因素。在这项工作中，合成了等电点（pI）分别显著低于和高于 4.8 的木质素羧酸盐（LC）和季铵化木质素羧酸盐（LCQ-x，x%：季铵化试剂与酶解木质素的质量比）。pI 显著低于或高于 4.8 的 LC 或 LCQ-x 降低了纤维素酶对木质素的非生产性吸附，但对纤维素酶活性有显著抑制作用，因此对木质纤维素的酶水解增强作用不显著。然而，pI 约为 4.8 的 LCQ-x 保留了纤维素酶活性，显著降低了纤维素酶的非生产性吸附，因此显著增强了酶水解。2 g/L 的 LC、LCQ-40（pI=5.0）和 LCQ-100（pI=9.2）分别将预处理桉树的酶解消化率从 35.2%提高到 53.4%、95.3%和 60.4%。此外，由于具有优异的 pH 响应性能，LCQ 在酶糖化后可有效回收。
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