本土野生蘑菇提取物对致病生物的抗菌活性测定
Determination of Antimicrobial Activity of Extracts of Indigenous Wild Mushrooms against Pathogenic Organisms.
作者信息
Gebreyohannes Gebreselema, Nyerere Andrew, Bii Christine, Berhe Sbhatu Desta
机构信息
Department of Biological and Chemical Engineering, Mekelle Institute of Technology, Mekelle University, Ethiopia.
Molecular Biology and Biotechnology, Pan African University, Institute for Basic Sciences, Technology, and Innovation; Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
出版信息
Evid Based Complement Alternat Med. 2019 Feb 18;2019:6212673. doi: 10.1155/2019/6212673. eCollection 2019.
OBJECTIVE
This study has investigated the antimicrobial activity of extracts of indigenous wild mushrooms against selected organisms.
METHODS
Thirty-five (35) indigenous wild mushrooms were collected from Arabuko-Sokoke and Kakamega National Reserve Forests, Kenya. All mushrooms were identified and their contents were extracted and screened for their antimicrobial activities against (clinical isolate) (ATCC 13883), (clinical isolate), (ATCC 27853), (ATCC 25923), MRSA (ATCC 33591), (clinical isolate), and (ATCC 90018) using tetrazolium microtiter plate bioassay method.
RESULTS
Of the 35 tested mushroom extracts, extracts of three (3) mushrooms, namely, spp. (Arabuko-Sokoke forest), , and spp. (Kakamega forest), have shown promising antimicrobial activities against the tested organisms. The (ATCC 25923), (ATCC 27853), and Methicillin-Resistant (MRSA) (ATCC 33591) were the most susceptible to chloroform extract of spp. collected from Arabuko-Sokoke forest. Of the tested organisms, (ATCC 25923) was the most susceptible whereas was the most resistant organism to the hot water extract of spp. collected from Arabuko-Sokoke forest. Chloroform extract of spp. has shown the highest antibacterial activity against (ATCC 25923), MRSA (ATCC 33591), and (ATCC 13883) but limited activity against . All extracts of the three wild mushrooms have shown the most antibacterial activities against (ATCC 25923).
CONCLUSION
The present study has shown that the extracts of the three wild mushrooms have shown promising antimicrobial activities against the tested organisms.
目的
本研究调查了本土野生蘑菇提取物对选定微生物的抗菌活性。
方法
从肯尼亚的阿伯库索科克和卡卡梅加国家保护区森林中采集了35种本土野生蘑菇。对所有蘑菇进行了鉴定，并提取其成分，采用四氮唑微量滴定板生物测定法筛选它们对（临床分离株）（ATCC 13883）、（临床分离株）、（ATCC 27853）、（ATCC 25923）、耐甲氧西林金黄色葡萄球菌（MRSA）（ATCC 33591）、（临床分离株）和（ATCC 90018）的抗菌活性。
结果
在35种测试蘑菇提取物中，三种蘑菇的提取物，即阿伯库索科克森林的 spp.、和卡卡梅加森林的 spp.，对测试微生物显示出有前景的抗菌活性。从阿伯库索科克森林采集的 spp. 的氯仿提取物对（ATCC 25923）、（ATCC 27853）和耐甲氧西林金黄色葡萄球菌（MRSA）（ATCC 33591）最敏感。在测试微生物中，（ATCC 25923）最敏感，而对从阿伯库索科克森林采集的 spp. 的热水提取物最具抗性。 spp. 的氯仿提取物对（ATCC 25923）、MRSA（ATCC 33591）和（ATCC 13883）显示出最高的抗菌活性，但对活性有限。这三种野生蘑菇的所有提取物对（ATCC 25923）显示出最强的抗菌活性。
结论
本研究表明，这三种野生蘑菇的提取物对测试微生物显示出有前景的抗菌活性。
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