Laboratory of Epigenome Biology, Systems Biology Center, NHLBI, NIH, Bethesda, MD, USA.
National Institute of Health Sciences, Kawasaki, Japan.
Nat Methods. 2019 Apr;16(4):323-325. doi: 10.1038/s41592-019-0361-7. Epub 2019 Mar 28.
Our method for analyzing histone modifications, scChIC-seq (single-cell chromatin immunocleavage sequencing), involves targeting of the micrococcal nuclease (MNase) to a histone mark of choice by tethering to a specific antibody. Cleaved target sites are then selectively PCR amplified. We show that scChIC-seq reliably detects H3K4me3 and H3K27me3 target sites in single human white blood cells. The resulting data are used for clustering of blood cell types.
我们分析组蛋白修饰的方法,scChIC-seq(单细胞染色质免疫切割测序),涉及通过与特定抗体连接将微球菌核酸酶 (MNase) 靶向到所选组蛋白标记物,然后对切割的靶位点进行选择性 PCR 扩增。我们表明,scChIC-seq 可在单个人类白细胞中可靠地检测到 H3K4me3 和 H3K27me3 靶位点。所得数据用于聚类血细胞类型。