通过键能转移盒进行近红外生物发光成像。
Near-Infrared Bioluminescence Imaging with a through-Bond Energy Transfer Cassette.
机构信息
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, 223-8522, Japan.
Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo, 153-8902, Japan.
出版信息
Chembiochem. 2019 Aug 1;20(15):1919-1923. doi: 10.1002/cbic.201900149. Epub 2019 Jul 2.
A coelenterazine (CTZ) analogue emitting near-infrared (NIR) bioluminescence was synthesized for through-bond energy transfer (TBET)-based imaging modalities. The analogue, named Cy5-CTZ, was prepared by conjugating cyanine-5 (Cy5) dye to CTZ through an acetylene linker. This novel derivative is intrinsically fluorescent and emits NIR-shifted luminescence upon reacting with an appropriate luciferase, the Renilla luciferase. This Cy5-CTZ substrate is optically stable in physiological samples and rapidly permeabilize through the plasma membrane into the cytosolic compartment of live cells.
一种能发射近红外（NIR）生物荧光的腔肠素（CTZ）类似物被合成出来，用于基于键间能量转移（TBET）的成像模式。该类似物名为 Cy5-CTZ，通过乙炔连接子将 Cy5 染料连接到 CTZ 上制备得到。这种新型衍生物具有内在荧光性，与合适的荧光素酶——海肾荧光素酶反应后会发射出近红外位移的荧光。这种 Cy5-CTZ 底物在生理样本中具有光学稳定性，并且能够快速穿透质膜进入活细胞的胞质腔。
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