牛磺酸对人宫颈癌细胞增殖和凋亡的影响及其机制。
Impact of Taurine on the proliferation and apoptosis of human cervical carcinoma cells and its mechanism.
机构信息
Department of Biochemistry and Molecular Biology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, China.
Department of Pharmacy, Shangrao People's Hospital, Shangrao, Jiangxi 334003, China.
出版信息
Chin Med J (Engl). 2019 Apr 20;132(8):948-956. doi: 10.1097/CM9.0000000000000162.
BACKGROUND
Cervical cancer has the fourth highest incidence and mortality rate of all cancers in women worldwide; it seriously harms their physical and mental health. The aim of this study was to observe the roles and preliminary mechanism of Taurine (Tau)-induced apoptosis in cervical cancer cells.
METHODS
Cells from the human cervical cancer cell line SiHa were transfected with the recombinant plasmid pEGFP-N1-MST1 (mammalian sterile 20-like kinase 1); then, the cell proliferation activity was analyzed by the MTT assay, cell apoptosis by flow cytometry, and the related protein levels by Western blotting.
RESULTS
Tau inhibited the proliferation of SiHa cells and induced apoptosis in these cells (the apoptotic rate was 21.95% in the Tau 160 mmol/L group and 30% in the Tau 320 mmol/L group), upregulated the expression of the MST1 (control, 0.53; Tau 40-320 mmol/L groups, 0.84-1.45) and Bax (control, 0.45; Tau 40-320 mmol/L groups, 0.64-1.51) proteins (P < 0.01), and downregulated the expression of Bcl-2 (control, 1.28, Tau 40-320 mmol/L groups, 0.93-0.47) (P < 0.01). The overexpression of MST1 promoted the apoptosis of SiHa cells, enhanced the apoptosis-inductive effects of Tau (P < 0.01), upregulated the expression of the proapoptotic proteins p73, p53, PUMA (p53 upregulated modulator of apoptosis), and caspase-3, and promoted the phosphorylation of YAP (Yes-associated protein).
CONCLUSIONS
Tau inhibited the proliferation and induced the apoptosis of cervical cancer SiHa cells. The MST1 protein plays an important role in the Tau-induced apoptosis of cervical cancer cells.
背景
宫颈癌是全球女性癌症中发病率和死亡率第四高的癌症，严重危害她们的身心健康。本研究旨在观察牛磺酸（Tau）诱导宫颈癌细胞凋亡的作用及初步机制。
方法
将重组质粒 pEGFP-N1-MST1（哺乳动物 sterile 20 样激酶 1）转染入人宫颈癌 SiHa 细胞系，通过 MTT 检测分析细胞增殖活性，流式细胞术检测细胞凋亡，Western blot 检测相关蛋白水平。
结果
Tau 抑制 SiHa 细胞增殖并诱导细胞凋亡（Tau 160 mmol/L 组凋亡率为 21.95%，Tau 320 mmol/L 组凋亡率为 30%），上调 MST1（对照组 0.53；Tau 40-320 mmol/L 组 0.84-1.45）和 Bax（对照组 0.45；Tau 40-320 mmol/L 组 0.64-1.51）蛋白表达（P<0.01），下调 Bcl-2 蛋白表达（对照组 1.28，Tau 40-320 mmol/L 组 0.93-0.47）（P<0.01）。过表达 MST1 促进 SiHa 细胞凋亡，增强 Tau 的促凋亡作用（P<0.01），上调促凋亡蛋白 p73、p53、PUMA（凋亡上调 p53 调制物）和 caspase-3 的表达，促进 YAP（Yes 相关蛋白）磷酸化。
结论
Tau 抑制宫颈癌 SiHa 细胞增殖并诱导其凋亡，MST1 蛋白在 Tau 诱导宫颈癌细胞凋亡中起重要作用。
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