低容量柠檬酸盐管对一线止血检测结果的影响。

Impact of low volume citrate tubes on results of first-line hemostasis testing.

机构信息

Section of Clinical Biochemistry, University of Verona, Verona, Italy.

Department of Haematology, Sydney Centres for Thrombosis and Haemostasis, Institute of Clinical Pathology and Medical Research, NSW Health Pathology, Westmead Hospital, Westmead, NSW, Australia.

出版信息

Int J Lab Hematol. 2019 Aug;41(4):472-477. doi: 10.1111/ijlh.13028. Epub 2019 Apr 15.

DOI:10.1111/ijlh.13028

PMID:30985988

Abstract

INTRODUCTION

Pediatric tubes are increasingly used for drawing blood for hemostasis testing. This study has investigated the potential impact of low volume citrate tubes on results of first-line hemostasis testing.

METHODS

The study population comprised 34 patients on warfarin therapy and 17 ostensibly healthy volunteers. Blood was collected into five different evacuated blood tubes from each subject. On right arm, blood was drawn directly into two standard evacuated blood tubes (3-mL Vacuette and 2-mL Vacutest) and one evacuated low volume blood tube (1-mL Vacuette) by straight needle venipuncture. On left arm, blood was drawn using a 5-mL syringe and then transferred within two nonevacuated microtubes (0.5 mL MiniCollect and 0.5 mL Micro Test). Prothrombin time (PT), activated partial thromboplastin time (APTT), and fibrinogen were assayed on ACL TOP 700.

RESULTS

Spearman's correlation of PT, APTT, and fibrinogen values obtained using different tubes was always satisfactory (ie, ≥0.93). A statistically significant bias was frequently found by comparing values obtained in different tubes. Nevertheless, the minimum quality specifications for bias were exceeded only by comparing data of Vacuette 1 mL with those of all other blood tubes for PT, by comparing data of Micro Test 0.5 mL with those of all other blood tubes for APTT, and by comparing data of Micro Test 0.5-mL blood tubes with those of Vacuette 3 mL and Vacuette 1.

CONCLUSION

First-line hemostasis testing using low volume citrate tubes may display differences sometimes exceeding the minimum quality specifications.

摘要

简介

儿科用管越来越多地用于抽取血液进行止血检测。本研究调查了低容量柠檬酸盐管对一线止血检测结果的潜在影响。

方法

研究人群包括 34 名华法林治疗患者和 17 名表面健康志愿者。每位受试者的右臂均通过直针静脉穿刺直接采集到两种标准的真空采血管（3-mL Vacuette 和 2-mL Vacutest）和一种真空采血管（1-mL Vacuette）。在左臂，使用 5-mL 注射器抽取血液，然后将其转移到两个非真空采血管（0.5-mL MiniCollect 和 0.5-mL Micro Test）中。使用 ACL TOP 700 检测凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）和纤维蛋白原。

结果

使用不同采血管获得的 PT、APTT 和纤维蛋白原值的斯皮尔曼相关性始终令人满意（即≥0.93）。通过比较不同采血管获得的值，经常发现有统计学意义的偏差。然而，仅通过比较 Vacuette 1-mL 与所有其他采血管的 PT 数据、比较 Micro Test 0.5-mL 与所有其他采血管的 APTT 数据以及比较 Micro Test 0.5-mL 采血管与 Vacuette 3-mL 和 Vacuette 1-mL 采血管的数据，才发现数据偏差超过了最小质量规范。