（某种物质）的免疫组织化学定位及其对木薯叶片的形态学影响。（这里原文中“of”后面缺少具体内容，翻译时根据语境补充了“某种物质”，以使句子完整通顺）

Immunohistochemical localization of and its morphological effect on cassava leaves.

作者信息

Saggaf Maliha H, Ndunguru Joseph, Tairo Fred, Sseruwagi Peter, Ascencio-Ibáñez José Trino, Kilalo Dora, Miano Douglas W

机构信息

Mikocheni Agricultural Research Institute, P.O. Box, 6226, Dar-es-Salaam, Tanzania.

North Carolina State University, Raleigh, NC, 27695, USA.

出版信息

Physiol Mol Plant Pathol. 2019 Jan;105:67-76. doi: 10.1016/j.pmpp.2018.06.001.

The localization of (CBSV) in cassava () leaf tissues was determined and cellular morphological changes in CBSV-infected tissues were evaluated. CBSV-symptomatic leaves were screened with CBSV-specific primers using reverse-transcriptase polymerase chain reaction. Immunohistochemical reactions showed precipitation in CBSV-infected but not CBSV-free tissues, demonstrating successful localization of CBSV. Microscopic inspection showed significantly larger ( < 0.001) midribs in CBSV-infected compared with control (uninfected) leaves. Viral accumulation occurred in middle and lower but rarely in young upper leaves. This immunohistochemical method for virus localization will be invaluable for efficient screening of CBSV and for breeding resistant cassava.

确定了木薯褐色条纹病毒（CBSV）在木薯叶片组织中的定位，并评估了受CBSV感染组织中的细胞形态变化。使用逆转录聚合酶链反应，用CBSV特异性引物筛选出现CBSV症状的叶片。免疫组织化学反应显示，在受CBSV感染的组织中有沉淀，而在未感染CBSV的组织中则没有，这证明了CBSV的成功定位。显微镜检查显示，与对照（未感染）叶片相比，受CBSV感染的叶片中脉明显更宽（P＜0.001）。病毒在中部和下部叶片中积累，但在幼嫩的上部叶片中很少积累。这种用于病毒定位的免疫组织化学方法对于高效筛选CBSV和培育抗木薯品种将具有重要价值。