年轻小鼠与老年小鼠间非清髓性同基因骨髓移植后最大寿命的延长及高骨髓嵌合率
Extension of Maximal Lifespan and High Bone Marrow Chimerism After Nonmyeloablative Syngeneic Transplantation of Bone Marrow From Young to Old Mice.
作者信息
Kovina Marina V, Karnaukhov Alexey V, Krasheninnikov Mikhail E, Kovin Artem L, Gazheev Sarul T, Sergievich Larisa A, Karnaukhova Elena V, Bogdanenko Elena V, Balyasin Maxim V, Khodarovich Yury M, Dyuzheva Tatyana G, Lyundup Alexey V
机构信息
Institute for Regenerative Medicine, Sechenov University, Moscow, Russia.
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia.
出版信息
Front Genet. 2019 Apr 12;10:310. doi: 10.3389/fgene.2019.00310. eCollection 2019.
The goal of this work was to determine the effect of nonablative syngeneic transplantation of young bone marrow (BM) to laboratory animals (mice) of advanced age upon maximum duration of their lifespan. To do this, transplantation of 100 million nucleated cells from BM of young syngeneic donors to an old nonablated animal was performed at the time when half of the population had already died. As a result, the maximum lifespan (MLS) increased by 28 ± 5%, and the survival time from the beginning of the experiment increased 2.8 ± 0.3-fold. The chimerism of the BM 6 months after the transplantation was 28%.
这项工作的目标是确定将年轻骨髓（BM）同基因非清髓性移植到老年实验动物（小鼠）体内对其最大寿命时长的影响。为此，在一半实验动物已经死亡时，将来自年轻同基因供体骨髓的1亿个有核细胞移植到未清髓的老年动物体内。结果，最大寿命（MLS）增加了28±5%，从实验开始起的存活时间增加了2.8±0.3倍。移植后6个月骨髓的嵌合率为28%。
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