Zekeridou Alkisti, Mombelli Andrea, Cancela Jose, Courvoisier Delphine, Giannopoulou Catherine
Division of Periodontology University Clinic of Dental Medicine, University of Geneva Geneva Switzerland.
Clin Exp Dent Res. 2019 Jan 24;5(2):128-135. doi: 10.1002/cre2.162. eCollection 2019 Apr.
In periodontitis patients, high levels of several inflammatory markers may be expressed in serum, reflecting the effect of local disease on the general health. The objective of the present analysis was to compare cytokine levels assessed in peripheral blood with those in the gingival crevicular fluid (GCF) and evaluate the impact of nonsurgical periodontal therapy on the incidence of high levels of 12 biomarkers in serum. Twenty-four patients with chronic periodontitis (Group P) contributed with serum and GCF samples at baseline (BL) and 1 and 3 months after periodontal treatment (M1 and M3). Samples were assessed for 12 cytokines using the Bio-Plex bead array multianalyte detection system. For each analyte, peak values were calculated as greater than the mean + 2 of the one found in 60 periodontally healthy participants. Significant correlations between serum and GCF values were obtained in the periodontitis group for interleukin (IL) 1ra, IL-6, and interferon γ at BL and for macrophage inflammatory protein 1β at M3 after treatment. Periodontitis subjects were found to exhibit high peaks for several inflammatory markers in serum. The highest incidence of peaks at BL was found for interferon γ (37.5% of the periodontitis subjects). For the four biomarkers with a detection frequency of >75% at BL (IL-1ra, IL-8, macrophage inflammatory protein 1β, and vascular endothelial growth factor), no significant difference was observed over time for the P group or between the two groups at BL. The significant correlation found between the serum and the GCF for certain cytokines and the fact that periodontitis subjects exhibit high peaks for several inflammatory markers in serum may support the hypothesis that the inflammatory reaction due to periodontitis is not restricted to the diseased sites. Within the limitations of the study, periodontal therapy did not seem to have any significant impact on the systemic cytokine levels.
在牙周炎患者中，血清中可能会表达多种高水平的炎症标志物，这反映了局部疾病对全身健康的影响。本分析的目的是比较外周血中评估的细胞因子水平与龈沟液（GCF）中的细胞因子水平，并评估非手术牙周治疗对血清中12种生物标志物高水平发生率的影响。24例慢性牙周炎患者（P组）在基线（BL）以及牙周治疗后1个月和3个月（M1和M3）提供了血清和GCF样本。使用Bio-Plex磁珠阵列多分析物检测系统对样本进行12种细胞因子的评估。对于每种分析物，峰值被计算为大于60名牙周健康参与者中所发现均值加2的值。在牙周炎组中，治疗前基线时白细胞介素（IL）-1ra、IL-6和干扰素γ以及治疗后M3时巨噬细胞炎性蛋白1β的血清和GCF值之间存在显著相关性。发现牙周炎患者血清中几种炎症标志物呈现高峰值。基线时干扰素γ的峰值发生率最高（占牙周炎患者的37.5%）。对于在基线时检测频率>75%的四种生物标志物（IL-1ra、IL-8、巨噬细胞炎性蛋白1β和血管内皮生长因子），P组随时间未观察到显著差异，两组在基线时也无显著差异。血清和GCF中某些细胞因子之间的显著相关性以及牙周炎患者血清中几种炎症标志物呈现高峰值这一事实，可能支持以下假设：牙周炎引起的炎症反应不仅限于患病部位。在本研究的局限性内，牙周治疗似乎对全身细胞因子水平没有任何显著影响。
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