DnaJB6 是一种 RanGTP 调节蛋白，在有丝分裂过程中对于微管组织是必需的。
DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis.
机构信息
Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology, Dr Aiguader 88, 08003 Barcelona, Spain.
Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology, Dr Aiguader 88, 08003 Barcelona, Spain
出版信息
J Cell Sci. 2019 Jun 3;132(11):jcs227033. doi: 10.1242/jcs.227033.
Bipolar spindle organization is essential for the faithful segregation of chromosomes during cell division. This organization relies on the collective activities of motor proteins. The minus-end-directed dynein motor complex generates spindle inward forces and plays a major role in spindle pole focusing. The dynactin complex regulates many dynein functions, increasing its processivity and force production. Here, we show that DnaJB6 is a novel RanGTP-regulated protein. It interacts with the dynactin subunit p150Glued (also known as DCTN1) in a RanGTP-dependent manner specifically in M-phase, and promotes spindle pole focusing and dynein force generation. Our data suggest a novel mechanism by which RanGTP regulates dynein activity during M-phase.
双极纺锤体组织对于细胞分裂过程中染色体的正确分离至关重要。这种组织依赖于马达蛋白的集体活动。负向导向的动力蛋白复合物产生纺锤体向内的力，并在纺锤体极聚焦中发挥主要作用。动力蛋白复合物调节许多动力蛋白的功能，增加其运动性和产生力的能力。在这里，我们表明 DnaJB6 是一种新型的 RanGTP 调节蛋白。它以 RanGTP 依赖性方式与动力蛋白复合物亚基 p150Glued（也称为 DCTN1）相互作用，这种相互作用仅在有丝分裂期发生，并促进纺锤体极聚焦和动力蛋白力的产生。我们的数据表明，RanGTP 在有丝分裂期间调节动力蛋白活性的一种新机制。
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