Department of Sports Medicine and Sports Nutrition, Ruhr University Bochum, 44801 Bochum, Germany.
Medizinisches Proteom-Center, Medical Faculty, Ruhr University Bochum, 44801 Bochum, Germany.
Nutrients. 2019 May 14;11(5):1072. doi: 10.3390/nu11051072.
Evidence has shown that protein supplementation following resistance exercise training (RET) helps to further enhance muscle mass and strength. Studies have demonstrated that collagen peptides containing mostly non-essential amino acids increase fat-free mass (FFM) and strength in sarcopenic men. The aim of this study was to investigate whether collagen peptide supplementation in combination with RET influences the protein composition of skeletal muscle. Twenty-five young men (age: 24.2 ± 2.6 years, body mass (BM): 79.6 ± 5.6 kg, height: 185.0 ± 5.0 cm, fat mass (FM): 11.5% ± 3.4%) completed body composition and strength measurements and vastus lateralis biopsies were taken before and after a 12-week training intervention. In a double-blind, randomized design, subjects consumed either 15 g of specific collagen peptides (COL) or a non-caloric placebo (PLA) every day within 60 min after their training session. A full-body hypertrophy workout was completed three times per week and included four exercises using barbells. Muscle proteome analysis was performed by liquid chromatography tandem mass spectrometry (LC-MS/MS). BM and FFM increased significantly in COL compared with PLA, whereas no differences in FM were detected between the two groups. Both groups improved in strength levels, with a slightly higher increase in COL compared with PLA. In COL, 221 higher abundant proteins were identified. In contrast, only 44 proteins were of higher abundance in PLA. In contrast to PLA, the upregulated proteins in COL were mostly associated with the protein metabolism of the contractile fibers. In conclusion, the use of RET in combination with collagen peptide supplementation results in a more pronounced increase in BM, FFM, and muscle strength than RET alone. More proteins were upregulated in the COL intervention most of which were associated with contractile fibers.
证据表明，抗阻运动训练（RET）后补充蛋白质有助于进一步增强肌肉质量和力量。研究表明，含有大多数非必需氨基酸的胶原蛋白肽可增加肌少症男性的去脂体重（FFM）和力量。本研究旨在探讨胶原蛋白肽补充与 RET 结合是否会影响骨骼肌的蛋白质组成。25 名年轻男性（年龄：24.2±2.6 岁，体重（BM）：79.6±5.6kg，身高：185.0±5.0cm，体脂（FM）：11.5%±3.4%）完成了身体成分和力量测量，并在 12 周训练干预前后采集了股外侧肌活检。在一项双盲、随机设计中，受试者每天在训练后 60 分钟内分别服用 15 克特定胶原蛋白肽（COL）或无热量安慰剂（PLA）。每周进行三次全身增肌训练，包括四项杠铃练习。采用液相色谱串联质谱（LC-MS/MS）进行肌肉蛋白质组分析。与 PLA 相比，COL 组的 BM 和 FFM 显著增加，而两组之间的 FM 没有差异。两组的力量水平都有所提高，COL 组的提高略高于 PLA 组。在 COL 组中，鉴定出 221 种高丰度蛋白质。相比之下，PLA 中只有 44 种蛋白质丰度较高。与 PLA 相比，COL 中上调的蛋白质主要与收缩纤维的蛋白质代谢有关。总之，与单独使用 RET 相比，RET 结合胶原蛋白肽补充可导致 BM、FFM 和肌肉力量的增加更为明显。COL 干预中上调的蛋白质更多，其中大多数与收缩纤维有关。
Zotero 插件
