Chitinophaga lutea sp. nov., isolated from arsenic-contaminated soil.

A yellow-coloured bacterial strain, designated ZY74, was isolated from arsenic contaminated soil (34 mg kg) sample collected in Longkou, Hubei Province, PR China. Cells were Gram-stain-negative, aerobic, non-motile and rod-shaped. Strain ZY74 produced round, yellow-pigmented, smooth and opaque colonies. Based on the results of 16S rRNA gene sequence analysis, strain ZY74 was found to be affiliated with members of the genus Chitinophaga. Its closest members were Chitinophagabarathri YLT18 (97.72 %) and Chitinophaganiabensis JS13-10 (97.17 %). The genome size of strain ZY74 was 6.61 Mb, containing 5351 predicted protein-coding genes, with a DNA G+C content of 55.0 mol%. The average nucleotide identity values of strain ZY74 with C. barathri YLT18 and C. niabensis DSM 24787 were 76.12 and 73.32 %, respectively. The digital DNA-DNA hybridization values of strain ZY74 with C. barathri YLT18 and C. niabensis JS13-10 were 20.60 and 19.40 %, respectively. The major respiratory quinone was menaquinone 7 and the predominant fatty acids (>5 %) were iso-C15:0, C16 : 1ω5c and iso-C17 : 03-OH. On the basis of phylogenetic, genotypic, phenotypic and chemotaxonomic characterization, strain ZY74 represents a novel species in the genus Chitinophaga, for which the name Chitinophagalutea sp. nov. is proposed. The type strain is ZY74 (=CCTCC AB2018369=KCTC 72039).