单剂量阿司匹林对体内血栓素A2和前列环素环氧合酶的影响。

Effect of single-dose aspirin on TXA2 and PGI2 cyclooxygenases in vivo.

作者信息

Zaragoza R, Le Breton G C

出版信息

Haemostasis. 1987;17(1-2):40-8. doi: 10.1159/000215557.

Abstract

The present study investigated the sensitivities of the thromboxane A2 (TXA2) cyclooxygenase and the prostacyclin (PGI2) cyclooxygenase to aspirin using an in vivo animal model. In this model, arachidonic acid (AA) was administered to mice via cardiac puncture, and plasma levels of thromboxane B2 (TXB2) and 6-keto prostaglandin F1 alpha (6-keto PGF1 alpha) were determined. Infusion of AA (5, 10, 25 and 50 mg/kg) resulted in a dose-dependent increase in both TXB2 and 6-keto PGF1 alpha. Pretreatment with aspirin resulted in a dose-dependent and parallel decrease in TXB2 and 6-keto PGF1 alpha production. This nonselective inhibition occurred at all doses of aspirin (1, 10 and 50 mg/kg) and at all levels of cyclooxygenase activity (5-50 mg/kg AA). These results indicate that the TXA2 and PGI2 cyclooxygenase enzymes are equally sensitive to inhibition by a single dose of aspirin.

摘要

本研究使用体内动物模型，研究了血栓素A2（TXA2）环氧化酶和前列环素（PGI2）环氧化酶对阿司匹林的敏感性。在该模型中，通过心脏穿刺向小鼠给予花生四烯酸（AA），并测定血浆中血栓素B2（TXB2）和6-酮-前列腺素F1α（6-酮-PGF1α）的水平。输注AA（5、10、25和50mg/kg）导致TXB2和6-酮-PGF1α均呈剂量依赖性增加。阿司匹林预处理导致TXB2和6-酮-PGF1α生成呈剂量依赖性且平行下降。这种非选择性抑制在所有剂量的阿司匹林（1、10和50mg/kg）以及所有环氧化酶活性水平（5-50mg/kg AA）下均会发生。这些结果表明，TXA2和PGI2环氧化酶对单剂量阿司匹林的抑制同样敏感。