利用肉眼进行疾病标志物的定量评估：基于气体生成的生物传感器免疫层析条的即时检测。

Quantitative assessment of disease markers using the naked eye: point-of-care testing with gas generation-based biosensor immunochromatographic strips.

机构信息

The First Affiliated Hospital, Biomedical Translational Research Institute and School of Pharmacy and, and Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou, 510632, China.

Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, 510632, People's Republic of China.

出版信息

J Nanobiotechnology. 2019 May 17;17(1):67. doi: 10.1186/s12951-019-0493-z.

DOI:10.1186/s12951-019-0493-z

PMID:31101112

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6524263/

Abstract

BACKGROUND

Immunochromatographic strips (ICSs) are a practical tool commonly used in point-of-care testing (POCT) applications. However, ICSs that are currently available have low sensitivity and require expensive equipment for quantitative analysis. These limitations prohibit their extensive use in areas where medical resources are scarce.

METHODS

We developed a novel POCT platform by integrating a gas generation biosensor with Au@Pt Core/Shell nanoparticle (Au@PtNPs)-based ICSs (G-ICSs). The resulting G-ICSs enabled the convenient and quantitative assessment of a target protein using the naked eye, without the need for auxiliary equipment or complicated computation. To assess this platform, C-reactive protein (CRP), a biomarker commonly used for the diagnosis of acute, infectious diseases was chosen as a proof-of-concept test.

RESULTS

The linear detection range (LDR) of the G-ICSs for CRP was 0.05-6.25 μg/L with a limit of detection (LOD) of 0.041 μg/L. The G-ICSs had higher sensitivity and wider LDR when compared with commonly used AuNPs and fluorescent-based ICSs. When compared with results from a chemiluminescent immunoassay, G-ICS concordance rates for CRP detection in serum samples ranged from 93.72 to 110.99%.

CONCLUSIONS

These results demonstrated that G-ICSs have wide applicability in family diagnosis and community medical institutions, especially in areas with poor medical resources.

摘要

背景

免疫层析条（ICSs）是一种常用于即时检测（POCT）应用的实用工具。然而，目前可用的 ICSs 灵敏度较低，需要昂贵的设备进行定量分析。这些限制限制了它们在医疗资源匮乏地区的广泛应用。

方法

我们通过将气体发生生物传感器与基于金@铂核/壳纳米粒子（Au@PtNPs）的免疫层析条（ICSs）（G-ICSs）集成，开发了一种新型的 POCT 平台。由此产生的 G-ICSs 使人们能够方便地使用肉眼对目标蛋白进行定量评估，而无需辅助设备或复杂的计算。为了评估该平台，我们选择 C-反应蛋白（CRP）作为概念验证测试，这是一种常用于诊断急性、感染性疾病的生物标志物。

结果

G-ICSs 对 CRP 的线性检测范围（LDR）为 0.05-6.25μg/L，检测限（LOD）为 0.041μg/L。与常用的金纳米颗粒和荧光免疫层析条相比，G-ICSs 具有更高的灵敏度和更宽的 LDR。与化学发光免疫分析的结果相比，G-ICSs 在血清样本中检测 CRP 的一致性率在 93.72%至 110.99%之间。