Aghaei Afsaneh, Moghbeli Majid, Kargar Mohammad, Nazarian Shahram, Kafilzadeh Farshid
Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran.
Department of Biology, Islamic Azad University, Damghan Branch, Damghan, Iran.
Biologicals. 2019 Jul;60:55-59. doi: 10.1016/j.biologicals.2019.05.003. Epub 2019 May 16.
Foot-and-mouth disease (FMD) is a highly contagious disease of livestock animals and control of the disease based on vaccination against serotypes O, A and Asia 1 is important. VP1 (structural) protein and 3A (non-structural) protein is the important antigen in FMDV and they can be used to design recombinant vaccines. In this study the bioinformatics characteristics of VP1 [141-160 and 23-42] and 3A [21-35] of Iranian serotypes O, A and Asia 1 was obtained using on-line predicting software. Then the sequence VP1 [141-160]-GS-VP1 [23-42]-GS-3A [21-35]-GS were codon-optimized and cloned onpHT43shuttle vector and finally expressed in Bacillus subtilis WB600 strain. We could predict VP1 [141-160] as a B cell epitope, VP1 [23-42] as a CTL epitope and 3A [21-35] as a Th cell epitope. The 20KD recombinant protein expressed by Bacillus subtilis were detected by SDS-PAGE. The results showed that this recombinant protein had epitope characteristics and it could be useful as a vaccine candidate to control all serotypes of FMD in Iran.
口蹄疫（FMD）是家畜的一种高度传染性疾病，基于针对O、A和亚洲1型血清型的疫苗接种来控制该疾病很重要。VP1（结构）蛋白和3A（非结构）蛋白是口蹄疫病毒中的重要抗原，它们可用于设计重组疫苗。在本研究中，使用在线预测软件获得了伊朗O、A和亚洲1型血清型的VP1[141 - 160和23 - 42]以及3A[21 - 35]的生物信息学特征。然后对序列VP1[141 - 160]-GS-VP1[23 - 42]-GS-3A[21 - 35]-GS进行密码子优化，并克隆到pHT43穿梭载体上，最后在枯草芽孢杆菌WB600菌株中表达。我们可以预测VP1[141 - 160]为B细胞表位，VP1[23 - 42]为CTL表位，3A[21 - 35]为Th细胞表位。通过SDS - PAGE检测枯草芽孢杆菌表达的20KD重组蛋白。结果表明，该重组蛋白具有表位特征，可作为控制伊朗所有口蹄疫血清型的候选疫苗。
Zotero 插件