日本血吸虫感染免疫诊断抗原的协作研究。
Collaborative study on antigens for immunodiagnosis of Schistosoma japonicum infection.
作者信息
Mott K E, Dixon H, Carter C E, Garcia E, Ishii A, Matsuda H, Mitchell G, Owhashi M, Tanaka H, Tsang V C
出版信息
Bull World Health Organ. 1987;65(2):233-44.
Six research laboratories in Australia, Japan, the Philippines and the USA participated in a collaborative evaluation of immunodiagnostic tests for Schistosoma japonicum infections. The serum bank consisted of 385 well-documented sera from Brazil, Kenya, Philippines, Republic of Korea and Europe. Twelve S. japonicum antigen/test system combinations were evaluated.Crude S. japonicum egg antigens showed the highest sensitivity and specificity. The defined or characterized antigens showed no advantage over the crude antigens. Quantitative seroreactivity of all S. japonicum antigens showed a positive correlation with faecal egg counts (log x + 1) in all age groups. The performance of the circumoval precipitin test was satisfactory within the same laboratory but with differences in the results between laboratories. A monoclonal antibody used in a competitive radioimmunoassay test system performed as well as the crude egg antigens.The high sensitivity of crude S. japonicum antigens now permits further evaluation for wide-scale use in public health laboratories of endemic areas to support control efforts.
澳大利亚、日本、菲律宾和美国的六个研究实验室参与了对日本血吸虫感染免疫诊断检测的合作评估。血清库由来自巴西、肯尼亚、菲律宾、韩国和欧洲的385份记录完备的血清组成。对12种日本血吸虫抗原/检测系统组合进行了评估。日本血吸虫粗卵抗原显示出最高的敏感性和特异性。经过定义或表征的抗原并未显示出比粗抗原更具优势。所有日本血吸虫抗原的定量血清反应性在所有年龄组中均与粪卵计数（log x + 1）呈正相关。环卵沉淀试验在同一实验室中的表现令人满意，但各实验室之间的结果存在差异。在竞争放射免疫分析检测系统中使用的单克隆抗体与粗卵抗原表现相当。日本血吸虫粗抗原的高敏感性现在允许在流行地区的公共卫生实验室中进一步评估其大规模使用，以支持防控工作。
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