Department of Advance Organic Materials and Textile System Engineering, Chungnam National University, Daejeon 34134, Korea.
Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon 34134, Korea.
Int J Nanomedicine. 2019 Apr 23;14:2773-2780. doi: 10.2147/IJN.S196472. eCollection 2019.
An important application of silver nanoparticles (Ag NPs) is their use as an antimicrobial and wound dressing material. The aim of this study is to investigate the morphological dependence on the antimicrobial activity and cellular response of Ag NPs. Ag NPs of various shapes were synthesized in an aqueous solution using a simple method. The morphology of the synthesized Ag NPs was observed via TEM imaging. The antimicrobial activity of the Ag NPs with different morphologies was evaluated against various microorganisms ( [] [] []). The antimicrobial activity of the Ag NPs was also examined according to the concentration in terms of the growth rate of . The TEM images indicated that the Ag NPs with different morphologies (sphere, disk and triangular plate) had been successfully synthesized. The antimicrobial activity obtained from the inhibition zone was in the order of spherical Ag NPs > disk Ag NPs > triangular plate Ag NPs. In contrast, fibroblast cells grew well in all types of Ag NPs when the cell viability was evaluated via an MTT assay. An inductively coupled plasma mass assay showed that the difference in the antimicrobial activities of the Ag NPs was closely associated with the difference in the release rate of the Ag ions due to the difference in the surface area of the Ag NPs. The morphological dependence of the antimicrobial activity of the Ag NPs can be explained by the difference in the Ag ion release depending on the shape. Therefore, it will be possible to control the antimicrobial activity by controlling the shape and size of the Ag NPs.
银纳米粒子（Ag NPs）的一个重要应用是将其用作抗菌剂和伤口敷料材料。本研究旨在研究 Ag NPs 的抗菌活性和细胞反应与形态的依赖关系。采用简单的方法在水溶液中合成了各种形状的 Ag NPs。通过 TEM 成像观察合成的 Ag NPs 的形态。用不同形态的 Ag NPs 对各种微生物（[] [] []）进行了抗菌活性评估。根据生长速率[]，还根据浓度研究了 Ag NPs 的抗菌活性。TEM 图像表明，已经成功合成了具有不同形态（球形、盘形和三角形板）的 Ag NPs。从抑菌圈获得的抗菌活性顺序为球形 Ag NPs > 盘形 Ag NPs > 三角形板 Ag NPs。相比之下，通过 MTT 测定评估细胞活力时，所有类型的 Ag NPs 中都有纤维母细胞生长良好。电感耦合等离子体质谱法（ICP-MS）表明，Ag NPs 的抗菌活性差异与 Ag NPs 表面积的差异密切相关，这与 Ag 离子释放率的差异有关。Ag NPs 的抗菌活性的形态依赖性可以通过 Ag 离子释放的形状依赖性来解释。因此，通过控制 Ag NPs 的形状和尺寸，有可能控制其抗菌活性。
