Serkova Natalie J, Davis Denise M, Steiner Jenna, Agarwal Rajesh
Department of Radiology, School of Medicine, University of Colorado Denver, Aurora, CO, USA.
Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Denver, Aurora, CO, USA.
Methods Mol Biol. 2019;1978:369-387. doi: 10.1007/978-1-4939-9236-2_23.
Nuclear magnetic resonance (NMR) spectroscopy is an established analytical platform for analyzing metabolic profiles of cells, tissues, and body fluids. There are several advantages in introducing an NMR-based study design into metabolomics studies, including a fast and comprehensive detection, characterization, and quantification of dozens of endogenous metabolites in a single NMR spectrum. Quantitative proton H-NMR is the most useful NMR-based platform for metabolomics. The frozen tissues can be analyzed noninvasively using a high-resolution magic angle spinning (HR-MAS) H-NMR spectroscopy; or several extraction techniques can be applied to detect additional metabolites using a conventional liquid-based NMR technique. In this chapter, we report on tissue collection, handling, extraction methods, and H-NMR acquisition protocols developed in the past decades for a precise and quantitative NMR-metabolomics approach. The NMR acquisition protocols (both HR-MAS and conventional H-NMR spectroscopy) and spectral analysis steps are also presented. Since NMR can be applied "in vivo" using horizontal bore MRI scanners, several in vivo sequences for localized H-MRS (magnetic resonance spectroscopy) are presented which can be directly applied for noninvasive detection of brain metabolites.
核磁共振（NMR）波谱法是一种成熟的分析平台，用于分析细胞、组织和体液的代谢谱。将基于NMR的研究设计引入代谢组学研究有几个优点，包括在单个NMR谱中对数十种内源性代谢物进行快速、全面的检测、表征和定量。定量质子H-NMR是代谢组学中最有用的基于NMR的平台。冷冻组织可以使用高分辨率魔角旋转（HR-MAS）H-NMR波谱法进行非侵入性分析；或者可以应用几种提取技术，使用传统的基于液体的NMR技术检测其他代谢物。在本章中，我们报告了过去几十年中开发的用于精确和定量NMR代谢组学方法的组织收集、处理、提取方法和H-NMR采集方案。还介绍了NMR采集方案（HR-MAS和传统H-NMR波谱法）以及光谱分析步骤。由于NMR可以使用水平孔径MRI扫描仪“体内”应用，因此介绍了几种用于局部H-MRS（磁共振波谱法）的体内序列，这些序列可直接用于脑代谢物的非侵入性检测。
