Foroutan Aidin, Goldansaz Seyed Ali, Lipfert Matthias, Wishart David S
Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada.
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada.
Methods Mol Biol. 2019;1996:311-324. doi: 10.1007/978-1-4939-9488-5_23.
Nuclear magnetic resonance (NMR) spectroscopy is widely considered to be one of the most robust and reproducible analytical platforms for conducting metabolomic experiments. As a metabolomic platform, NMR is not particularly sensitive, but it is nondestructive and requires no prior derivatization or chromatographic separation. It is also very automatable, easy to perform, and highly reproducible and can be used to accurately quantify dozens of metabolites in complex mixtures. To perform a successful NMR metabolomic experiment, it is important to follow good practices in sample preparation, data acquisition, and data analysis. In this chapter, we will describe, step-by-step, the preparation of different livestock samples, including both biofluids (whole blood, serum, urine, rumen content, and fecal water) and tissues (liver, muscle, testis). We will also describe the protocols for acquiring optimal NMR spectra and the techniques used to identify and quantify water-soluble metabolites by NMR spectroscopy.
核磁共振（NMR）光谱法被广泛认为是进行代谢组学实验最可靠且可重复的分析平台之一。作为一种代谢组学平台，NMR的灵敏度并不特别高，但它具有非破坏性，无需事先衍生化或色谱分离。它还具有很强的自动化能力，操作简便，重复性高，可用于准确量化复杂混合物中的数十种代谢物。要成功进行NMR代谢组学实验，在样品制备、数据采集和数据分析过程中遵循良好的规范非常重要。在本章中，我们将逐步描述不同家畜样品的制备方法，包括生物流体（全血、血清、尿液、瘤胃内容物和粪便水）和组织（肝脏、肌肉、睾丸）。我们还将描述获取最佳NMR光谱的方案以及通过NMR光谱法识别和量化水溶性代谢物的技术。
