基质金属蛋白酶 9 和基质金属蛋白酶组织抑制剂 1 在喉鳞状细胞癌中的表达及浓度。
Expression and Concentration of Matrix Metalloproteinase 9 and Tissue Inhibitor of Matrix Metalloproteinases 1 in Laryngeal Squamous Cell Carcinoma.
机构信息
Department of Otolaryngology, Medical University of Bialystok, Marii Skłodowskiej-Curie 24A, 15-276 Bialystok, Poland.
Department of Neurodegeneration Diagnostics, Medical University of Bialystok, Waszyngtona 15A, 15-269 Bialystok, Poland.
出版信息
Dis Markers. 2019 Apr 18;2019:3136792. doi: 10.1155/2019/3136792. eCollection 2019.
The aim of this study was to assess the expression of MMP-9 and TIMP-1 in cancerous tissue as well as in the serum and plasma concentrations of these proteins in patients with laryngeal cancer and compare the results to the inflammatory reaction in healthy subjects. Twenty-seven patients who were diagnosed with laryngeal carcinoma and selected for total laryngectomy were included in the study group. MMP-9 and TIMP-1 expression in tissues was assessed using immunohistochemical assays. Immunoenzymatic ELISA methods were used to measure MMP-9 and TIMP-1 concentrations in serum and plasma. MMP-9 and TIMP-1 were identified in tumor cells and in the tumor stroma compartment, as well as in macroscopically healthy mucous membrane. MMP-9 expression was more significant in tumor stroma than in the perimatrix of the mucous membrane ( = 0.047). TIMP-1 expression was significantly higher in the matrix and perimatrix of the mucous membrane than in cancer tissue ( = 0.0093) and the tumor stroma compartment ( < 0.0001). Expression of TIMP-1 was observed more frequently in tumors without infiltrated lymph nodes ( = 0.009). Serum concentrations of MMP-9 and TIMP-1 as well as plasma TIMP-1 concentration were significantly higher in the study group than in the control group ( = 0.0004, = 0.002, and = 0.0001, respectively). A significantly higher TIMP-1 level in plasma was found in patients with poorly differentiated tumors compared to G1 and G2 ( = 0.046). MMP-9/TIMP-1 rate in serum was significantly higher in the study group than in the control group. The balance between the level of MMP-9 and TIMP-1 is disrupted in laryngeal cancer. The significant correlation between TIMP-1 expression and the presence of lymph node metastases, as well as that between TIMP-1 plasma concentration and stage of cancer histological differentiation, might indicate the importance of this molecule as a prognostic factor during carcinogenesis.
本研究旨在评估 MMP-9 和 TIMP-1 在癌症组织中的表达，以及这些蛋白在喉癌患者血清和血浆中的浓度，并将结果与健康受试者的炎症反应进行比较。本研究纳入了 27 例经诊断为喉癌并选择行全喉切除术的患者作为研究组。使用免疫组织化学方法评估 MMP-9 和 TIMP-1 在组织中的表达。采用免疫酶联 ELISA 法测量血清和血浆中 MMP-9 和 TIMP-1 的浓度。MMP-9 和 TIMP-1 在肿瘤细胞和肿瘤基质部位以及宏观健康的黏膜中均有发现。肿瘤基质中 MMP-9 的表达显著高于黏膜周围基质（ = 0.047）。TIMP-1 在基质和黏膜周围基质中的表达显著高于癌组织（ = 0.0093）和肿瘤基质部位（ < 0.0001）。无淋巴结浸润的肿瘤中 TIMP-1 的表达更为常见（ = 0.009）。与对照组相比，研究组患者血清中 MMP-9 和 TIMP-1 以及血浆中 TIMP-1 的浓度明显更高（ = 0.0004、 = 0.002 和 = 0.0001）。与 G1 和 G2 相比，分化程度较差的肿瘤患者血浆中 TIMP-1 水平显著升高（ = 0.046）。研究组患者血清中 MMP-9/TIMP-1 比值明显高于对照组。喉癌中 MMP-9 和 TIMP-1 水平的平衡被打破。TIMP-1 表达与淋巴结转移的存在之间以及 TIMP-1 血浆浓度与癌症组织学分化阶段之间的显著相关性可能表明该分子作为癌发生过程中预后因素的重要性。
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