毛发皮质醇的自动化测定方法。
Hair Cortisol Measurement by an Automated Method.
机构信息
Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Bioquímica Clínica, Catedra de Bioquímica Clínica I, Buenos Aires, Argentina.
Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Instituto de Fisiopatología y Bioquímica Clínica (INFIBIOC), Buenos Aires, Argentina.
出版信息
Sci Rep. 2019 Jun 3;9(1):8213. doi: 10.1038/s41598-019-44693-3.
We present the development of the first procedure for hair cortisol measurement through an automated method. Hair samples were obtained from 286 individuals. After cortisol extraction, samples were measured in a Siemens Immulite 2000 (Gwynedd, UK) automated chemoluminiscent immunoassay analyzer. Normal reference values were obtained from hair cortisol levels measured in 213 healthy individuals with low levels of stress. Hair cortisol concentration median was 55 pg/mg hair (2.5-97.5 percentile (40-128)) in healthy individuals with low levels of stress and 250 pg/mg hair (range 182-520) in stressed individuals. No significant differences were observed in hair cortisol levels between subjects with and without dye (40 (40-107) and 40 (40-155) pg/mg hair, respectively; p = 0.128). The novel procedure presented here shows an adequate analytical performance.
我们提出了一种通过自动化方法测量头发皮质醇的新方法。从 286 个人中采集了头发样本。皮质醇提取后，将样本在西门子 Immulite 2000（英国格温内思）自动化化学发光免疫分析仪中进行测量。从 213 名压力水平较低的健康个体中测量的头发皮质醇水平获得了正常参考值。压力水平较低的健康个体的头发皮质醇浓度中位数为 55pg/mg 头发（2.5-97.5 百分位（40-128）），而压力个体的头发皮质醇浓度中位数为 250pg/mg 头发（范围 182-520）。在有和没有染发的个体之间，头发皮质醇水平没有显著差异（分别为 40（40-107）和 40（40-155）pg/mg 头发；p=0.128）。这里提出的新方法显示出良好的分析性能。
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