Key Laboratory of Tropical Biological Resources of Ministry Education, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, China.
School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China.
Appl Microbiol Biotechnol. 2019 Aug;103(15):6271-6285. doi: 10.1007/s00253-019-09878-w. Epub 2019 Jun 3.
Proton magnetic resonance-based metabolomics analysis was performed to determine the global metabolite changes in pathogenic bacterium Pseudomonas aeruginosa PAO1 following exposure to quorum sensing (QS) inhibitor hordenine. Pyocyanin inhibition assay confirmed that hordenine exhibited potent QS inhibitory activity. A total of 40 metabolites were assigned by PMR spectra. Hordenine treatment resulted in the destruction of QS system in P. aeruginosa PAO1 by downregulating the expressions of genes involved in QS. The synthesis of antioxidant enzymes was repressed and the oxidative stress was enhanced due to the dysfunctional QS system of P. aeruginosa PAO1. The enhanced oxidative stress induced by the dysfunctional QS system of P. aeruginosa PAO1 altered the membrane components, enhanced membrane permeability, and disturbed energy metabolism, amino acid metabolism, and nucleotide metabolism, and would ultimately attenuate the pathogenicity of P. aeruginosa PAO1. Hordenine may have promising potential for controlling nosocomial pathogens.
基于质子磁共振的代谢组学分析用于确定致病性细菌铜绿假单胞菌 PAO1 在暴露于群体感应（QS）抑制剂麦角碱后全球代谢物的变化。通过绿脓菌素抑制测定法证实麦角碱具有很强的 QS 抑制活性。通过 PMR 光谱共鉴定出 40 种代谢物。麦角碱处理通过下调参与 QS 的基因表达，破坏铜绿假单胞菌 PAO1 的 QS 系统。由于铜绿假单胞菌 PAO1 的 QS 系统功能失调，抗氧化酶的合成受到抑制，氧化应激增强。铜绿假单胞菌 PAO1 的功能失调的 QS 系统引起的增强的氧化应激改变了膜成分，增强了膜通透性，并扰乱了能量代谢、氨基酸代谢和核苷酸代谢，最终减弱了铜绿假单胞菌 PAO1 的致病性。麦角碱可能具有控制医院病原体的巨大潜力。
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