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在硅藻非无菌培养物中鉴定出的sp.菌株SMS9的基因组序列

Genome Sequence of sp. Strain SMS9 Identified in a Non-Axenic Culture of the Diatom .

作者信息

Pinder Matthew I M, Johansson Oskar N, Almstedt Alvar, Kourtchenko Olga, Clarke Adrian K, Godhe Anna, Töpel Mats

机构信息

Department of Marine Sciences, University of Gothenburg, Göteborg, Sweden.

Department of Biological and Environmental Sciences, University of Gothenburg, Göteborg, Sweden.

出版信息

J Genomics. 2019 May 9;7:46-49. doi: 10.7150/jgen.35061. eCollection 2019.

Abstract

Initial efforts to sequence the genome of the marine diatom were hampered by the presence of genetic material from bacteria, and there was sufficient material from some of these bacteria to enable the assembly of full chromosomes. Here, we report the genome of strain SMS9, one such bacterial species identified in a non-axenic culture of strain ST54. Its 5,482,391 bp circular chromosome contains 4,641 CDSs, and has a G+C content of 35.6%. Based on 16S rRNA comparison, phylotaxonomic analysis, and the genome similarity metrics dDDH and OrthoANI, we place this strain in the genus , and to the best of our knowledge, this is the first species to be initially described from European waters. As attempts to culture this strain have failed, however, the specifics of its relationship with are still uncertain.

摘要

对海洋硅藻基因组进行测序的初步努力受到细菌遗传物质的阻碍,并且来自其中一些细菌的物质足以组装完整的染色体。在此,我们报告菌株SMS9的基因组,这是在菌株ST54的非无菌培养物中鉴定出的一种此类细菌。其5,482,391 bp的环状染色体包含4,641个编码序列(CDS),G+C含量为35.6%。基于16S rRNA比较、系统分类分析以及基因组相似性指标dDDH和OrthoANI,我们将该菌株归入 属,据我们所知,这是首个最初从欧洲水域描述的 物种。然而,由于培养该菌株的尝试失败,其与 的关系细节仍不确定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/32a9/6536808/061afc3629b3/jgenv07p0046g001.jpg

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