Gujarat Pollution Control Board, Gandhinagar 382010, Gujarat, India.
Department of Microbiology, M. G. Science Institute, Ahmedabad 380009, Gujarat, India.
Bioresour Technol. 2019 Sep;288:121577. doi: 10.1016/j.biortech.2019.121577. Epub 2019 May 29.
This work was aimed to evaluate six qualitative and quantitative methods (hemolytic activity, cetyltrimethylammonium bromide agar plate method, oil spread method, drop collapse method, surface tension measurement and emulsification index) to study biosurfactant production by sixty-nine bacterial isolates which were obtained from petroleum crude contaminated soil and water samples. Among all isolates, Pseudomonas aeruginosa NCIM 5514 was evaluated as the most potent isolate producing rhamnolipid. Effectiveness of growth medium pH, inoculum size and concentration of NaCl on surface active properties and biomass & rhamnolipid production in fermentation medium were studied. Highest surface activity was obtained at 1% (v/v) inoculum at initial pH of the medium 7.2, which resulted 4.389 ± 0.019 and 3.146 ± 0.087 g/l biomass and rhamnolipid, respectively. Notable surface activity of rhamnolipid produced by P. aeruginosa NCIM 5514 makes it feasible to be used for industrial application.
本工作旨在评估六种定性和定量方法（溶血活性、溴化十六烷基三甲铵琼脂平板法、油扩散法、液滴坍塌法、表面张力测量和乳化指数），以研究从石油原油污染土壤和水样中获得的 69 个细菌分离物的生物表面活性剂生产情况。在所有分离物中，铜绿假单胞菌 NCIM 5514 被评估为生产鼠李糖脂的最有效分离物。研究了生长培养基 pH 值、接种量和 NaCl 浓度对发酵培养基中表面活性特性和生物量及鼠李糖脂生产的影响。在初始培养基 pH 值为 7.2、接种量为 1%（v/v）的情况下，表面活性最高，分别产生 4.389±0.019 和 3.146±0.087 g/l 的生物量和鼠李糖脂。铜绿假单胞菌 NCIM 5514 产生的鼠李糖脂具有显著的表面活性，使其可用于工业应用。
