2006-2015 年巴西肉牛和奶牛群中流行的轮状病毒 A 田间分离株的 G 和 P 基因型特征。
G and P genotype profiles of rotavirus A field strains circulating in beef and dairy cattle herds in Brazil, 2006-2015.
机构信息
Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina (UEL), Celso Garcia Cid Road, PR455 Km 380, P.O. Box 10011, CEP 86057-970, Londrina, Paraná, Brazil.
Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina (UEL), Celso Garcia Cid Road, PR455 Km 380, P.O. Box 10011, CEP 86057-970, Londrina, Paraná, Brazil; National Institute of Science and Technology for the Dairy Production Chain (INCT - Leite), Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina (UEL), Celso Garcia Cid Road, PR455 Km 380, P.O. Box 10011, CEP 86057-970, Londrina, Paraná, Brazil.
出版信息
Comp Immunol Microbiol Infect Dis. 2019 Jun;64:90-98. doi: 10.1016/j.cimid.2019.03.002. Epub 2019 Mar 8.
The aim of this retrospective study was to use RT-PCR and nucleotide sequencing analysis to determine the G (VP7 gene) and P (VP4 gene) genotypes of 155 Brazilian bovine rotavirus A (RVA) wild-type strains detected in diarrheic calves from all Brazilian geographical regions from 2006 to 2015. The RVA strains evaluated belonged to the G6, G10, P[5], and P[11] genotypes. The G6P[5] genotype was prevalent (65.5%; P < 0.05) in beef, and the G10P[11] (38.4%) and G6P[11] (30.8%) genotypes were more prevalent in dairy cattle herds. The Midwest was the region with the highest number of genotyped RVA strains, where the genotypes G6, P[5], and P[11] were identified. Genotype combination G6-IV/P[5]-IX, prevalent in beef herds, and G6-III/P[11]-III or G10-IV/P[11]-III, prevalent in dairy herds, were detected. In addition, for the first time in Brazil, we detected the P[5] and P[11] genotype RVA strains that belong to lineage II and VII, respectively.
本回顾性研究旨在使用 RT-PCR 和核苷酸测序分析来确定 2006 年至 2015 年期间巴西所有地理区域腹泻犊牛中检测到的 155 株巴西牛轮状病毒 A（RVA）野生型的 G（VP7 基因）和 P（VP4 基因）基因型。评估的 RVA 株属于 G6、G10、P[5]和 P[11]基因型。G6P[5]基因型在牛肉中较为流行（65.5%；P<0.05），而 G10P[11]（38.4%）和 G6P[11]（30.8%）基因型在奶牛群中更为流行。中西部是 RVA 基因分型株数量最多的地区，其中鉴定出了 G6、P[5]和 P[11]基因型。在肉牛群中流行的基因型组合 G6-IV/P[5]-IX 和在奶牛群中流行的 G6-III/P[11]-III 或 G10-IV/P[11]-III 被检测到。此外，我们首次在巴西检测到属于谱系 II 和 VII 的 P[5]和 P[11]基因型 RVA 株。
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