富含可水解单宁的热带饲料的产甲烷潜力 1,2。
Methanogenic potential of tropical feeds rich in hydrolyzable tannins1,2.
机构信息
Ecole Nationale Supérieure de Biotechnologie, Ali Mendjli, Constantine, Algeria.
†INRA, VetAgro Sup, UMR1213 Herbivores, Saint-Genès-Champanelle, France.
出版信息
J Anim Sci. 2019 Jul 2;97(7):2700-2710. doi: 10.1093/jas/skz199.
The present study was carried out to determine the effect of Acacia nilotica, a tropical plant rich in hydrolyzable tannins (HT), on rumen fermentation and methane (CH4) production in vitro. We used leaves and pods from A. nilotica alone and combined. The combination of HT from A. nilotica leaves and pods and condensed tannins (CT) from Calliandra calothyrsus and Leucaena leucocephala were also evaluated to assess potential differences in biological activity between HT and CT. Four series of 24-h incubations were performed using rumen contents of 4 sheep fed a tropical grass (natural grassland based on Dichanthium spp.). A first experiment tested different levels of replacement of this tropical forage (control [CTL] without tannins) by A. nilotica leaves or pods: 0:100, 25:75, 50:50, 75:25 and 100:0. A second experiment tested the mixture of A. nilotica leaves and pods in different proportions: 100:0, 75:25, 50:50, 25:75, and 0:100. A third experiment tested the 50:50 combination of A. nilotica leaves or pods with C. calothyrsus and L. leucocephala. Acacia nilotica pods and leaves had a high content of HT (350 and 178 g/kg DM, respectively), whereas C. calothyrsus and L. leucocephala had a high content of CT (361 and 180 g/kg DM, respectively). The inclusion of HT from A. nilotica leaves and pods decreased CH4 production dose-dependently (P < 0.01). Total replacement of the CTL by A. nilotica decreased CH4 production by 64 and 55% with leaves and pods, respectively. Pods were richer in HT than leaves, but their antimethanogenic effect did not differ (P > 0.05). Although A. nilotica leaves and pods inhibited fermentation, as indicated by the lower gas production and VFA production (P < 0.01), this effect was less pronounced than for CH4. Volatile fatty acid production decreased by 12% in leaves and by 30% in pods when compared with the CTL alone. Positive associative effect was reported for VFA, when HT-rich sources and CT-rich sources were mixed. Combining the 2 sources of HT did not show associative effects on fermentation or CH4 production (P > 0.05). Hydrolyzable tannin-rich sources were more effective in suppressing methanogenesis than CT-rich sources. Our results show that HT-rich A. nilotica leaves and pods have the potential to reduce ruminal CH4 production.
本研究旨在确定富含可水解单宁（HT）的热带植物金合欢（Acacia nilotica）对体外瘤胃发酵和甲烷（CH4）产生的影响。我们单独使用金合欢的叶子和豆荚，以及组合使用金合欢的叶子和豆荚中的 HT 与来自银合欢（Calliandra calothyrsus）和勒比朗相思（Leucaena leucocephala）的缩合单宁（CT）。还评估了 HT 和 CT 之间生物活性的潜在差异。使用 4 只绵羊的瘤胃液进行了 4 个系列 24 小时孵育，这些绵羊饲喂热带牧草（基于双花草的天然草地）。第一个实验测试了用金合欢的叶子或豆荚不同程度替代这种热带牧草（对照[CTL]不含单宁）：0:100、25:75、50:50、75:25 和 100:0。第二个实验测试了金合欢的叶子和豆荚以不同比例的混合物：100:0、75:25、50:50、25:75 和 0:100。第三个实验测试了金合欢的叶子或豆荚与银合欢和勒比朗相思的 50:50 组合。金合欢的叶子和豆荚含有高含量的 HT（分别为 350 和 178 g/kg DM），而银合欢和勒比朗相思含有高含量的 CT（分别为 361 和 180 g/kg DM）。金合欢叶子和豆荚中的 HT 含量与 CH4 产量呈剂量依赖性降低（P<0.01）。完全用金合欢替代 CTL 使 CH4 产量分别降低了 64%和 55%。豆荚中的 HT 含量高于叶子，但它们的抗甲烷作用没有差异（P>0.05）。尽管金合欢的叶子和豆荚抑制了发酵，如气体和 VFA 产量降低所示（P<0.01），但这种作用不如 CH4 明显。与单独的 CTL 相比，叶子中的 VFA 产量下降了 12%，豆荚中的 VFA 产量下降了 30%。当富含 HT 的来源与富含 CT 的来源混合时，观察到 VFA 呈正相关效应。将 2 种 HT 来源混合在一起对发酵或 CH4 产生没有表现出协同作用（P>0.05）。富含 HT 的来源在抑制甲烷生成方面比富含 CT 的来源更有效。我们的结果表明，富含 HT 的金合欢叶子和豆荚具有降低瘤胃 CH4 产生的潜力。
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