NANOG 通过激活 AKT 通路缓解 H2O2 对人毛囊间充质干细胞的损伤。
NANOG Alleviates the Damage of Human Hair Follicle Mesenchymal Stem Cells Caused by H2O2 through Activation of AKT Pathway.
机构信息
Department of Toxicology, School of Public Health, Jilin University, Changchun 130021, Jilin, China.
出版信息
Biomed Environ Sci. 2019 Apr;32(4):272-280. doi: 10.3967/bes2019.037.
OBJECTIVE
To explore the protective effect of NANOG against hydrogen peroxide (H2O2) -induced cell damage in the human hair follicle mesenchymal stem cells (hHF-MSCs).
METHODS
NANOG was expressed from a lentiviral vector, pLVX-IRES-ZsGreen. NANOG hHF-MSCs and vector hHF-MSCs were treated with 400 μmol/L hydrogen peroxide (H2O2) for 2 h, the cell survival rate, cell morphology, ROS production, apoptosis and expression of AKT, ERK, and p21 were determined and compared.
RESULTS
Our results showed that NANOG could activate AKT and upregulate the expression of p-AKT, but not p-ERK. When treated with 400 μmol/L H2O2, NANOG hHF-MSCs showed higher cell survival rate, lower ROS production and apoptosis, higher expression of p-AKT, higher ratio of p-AKT/AKT.
CONCLUSION
Our results suggest that NANOG could protect hHF-MSCs against cell damage caused by H2O2 through activating AKT signaling pathway.
目的
探讨 NANOG 对过氧化氢（H2O2）诱导的人毛囊间充质干细胞（hHF-MSCs）损伤的保护作用。
方法
利用慢病毒载体 pLVX-IRES-ZsGreen 表达 NANOG。用 400 μmol/L 过氧化氢（H2O2）处理 NANOG hHF-MSCs 和载体 hHF-MSCs 2 h，检测细胞存活率、细胞形态、ROS 生成、细胞凋亡以及 AKT、ERK 和 p21 的表达，并进行比较。
结果
结果表明，NANOG 可激活 AKT 并上调 p-AKT 的表达，但不影响 p-ERK 的表达。用 400 μmol/L H2O2 处理后，NANOG hHF-MSCs 的细胞存活率较高，ROS 生成和凋亡较低，p-AKT 表达较高，p-AKT/AKT 比值较高。
结论
NANOG 可通过激活 AKT 信号通路来保护 hHF-MSCs 免受 H2O2 引起的细胞损伤。
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