移植经基因改造的Nogo-B的人脐静脉内皮细胞可加速裸鼠伤口愈合。
Transplantation of HUVECs with genetically modified Nogo-B accelerates wound-healing in nude mice.
作者信息
Wang Xingtong, Zheng Yongjun, Wu Haibin, Tian Song, Wu Minjuan, Luo Pengfei, Zhang Fang, Fang He, Li Hengyu, Xia Zhaofan
机构信息
Department of Burns and Plastic Surgery, Sixth Medical Center of General Hospital, The People's Liberation Army Beijing 100046, China.
Burns Center of Changhai Hospital, The Second Military Medical University Shanghai 200433, China.
出版信息
Am J Transl Res. 2019 May 15;11(5):2866-2876. eCollection 2019.
Wound repair is an intractable problem in clinic, with limited treatment options. Previous studies have demonstrated the therapeutic potential of Nogo-B in tissue repairs. However, the therapeutic effect of Nogo-B in HUVEC is still unknown. In this study, we examined the benefit of genetically modified Human Umbilical Vein Endothelial Cells (HUVECs) and found that down regulation of Nogo-B significantly promoted secretion of growth factors involving in wound healing and greatly boosted the proliferation, migration of fibroblasts and epithelial cells. Moreover, using an excisional wound splinting model, we showed that injection of exogenous HUVEC-siNogo-B around the wound significantly enhanced angiogenesis and wound healing in nude mice. Thus, our data suggests that genetically modified HUVECs support microenvironment suitable for wound healing and systemically demonstrates the beneficial effect of HUVECs in wound healing.
伤口修复是临床上一个棘手的问题，治疗选择有限。先前的研究已经证明了Nogo-B在组织修复中的治疗潜力。然而，Nogo-B对人脐静脉内皮细胞（HUVEC）的治疗效果仍然未知。在本研究中，我们检测了基因改造的人脐静脉内皮细胞（HUVECs）的益处，发现下调Nogo-B可显著促进参与伤口愈合的生长因子的分泌，并极大地促进成纤维细胞和上皮细胞的增殖与迁移。此外，使用切除伤口夹板模型，我们发现向伤口周围注射外源性HUVEC-siNogo-B可显著增强裸鼠的血管生成和伤口愈合。因此，我们的数据表明，基因改造的HUVECs支持适合伤口愈合的微环境，并系统地证明了HUVECs在伤口愈合中的有益作用。
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