在睡眠剥夺的雌性大鼠中，规律的跑步机运动可改善海马体中异常的miR-1b表达。
Abnormal hippocampal miR-1b expression is ameliorated by regular treadmill exercise in the sleep-deprived female rats.
作者信息
Mohammadipoor-Ghasemabad Lily, Sangtarash Mohammad Hossein, Esmaeili-Mahani Saeed, Sheibani Vahid, Sasan Hosein Ali
机构信息
Department of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran.
Laboratory of Molecular Neuroscience, Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Science, Kerman, Iran.
出版信息
Iran J Basic Med Sci. 2019 May;22(5):485-490. doi: 10.22038/ijbms.2019.31988.7734.
OBJECTIVES
The protective effect of regular running on sleep deprivation (SD)-induced cognitive impairment has been revealed. In this study, we focused on the effects of regular exercise, sleep deprivation and both of them together on the microRNA-1b (miR-1b) expression and their relation to the behavioral parameters and brain-derived neurotrophic factor (BDNF) expression.
MATERIALS AND METHODS
We used ovariectomized (OVX) female rats. The exercise program was mild-moderate treadmill training for 4 weeks. 72 hr SD was achieved using the multiple platform method and the spatial learning and memory parameters have been evaluated by the Morris water maze (MWM) test. The levels of studied genes were quantified by real-time PCR.
RESULTS
SD down-regulated pri-miR-1b, miR-1b (˂0.05), and BDNF mRNA (˂0.01) in the hippocampus. Furthermore, female rats under exercise conditions showed significant up-regulation of the miR-1b and BDNF mRNA (˂0.001). In addition, miR-1b positively correlated with cognitive function (˂0.05) and BDNF mRNA (˂0.01).
CONCLUSION
Our data demonstrated that regular treadmill exercise could reverse the down-regulation of hippocampal miR-1b, which has a probable role in the SD-induced cognitive impairment.
目的
规律跑步对睡眠剥夺（SD）诱导的认知障碍的保护作用已被揭示。在本研究中，我们重点关注规律运动、睡眠剥夺以及二者共同作用对微小RNA-1b（miR-1b）表达的影响，及其与行为参数和脑源性神经营养因子（BDNF）表达的关系。
材料与方法
我们使用去卵巢（OVX）雌性大鼠。运动方案为轻度至中度跑步机训练4周。采用多平台法实现72小时睡眠剥夺，并通过莫里斯水迷宫（MWM）试验评估空间学习和记忆参数。通过实时PCR对研究基因的水平进行定量。
结果
睡眠剥夺下调了海马体中的初级miR-1b、miR-1b（<0.05）和BDNF mRNA（<0.01）。此外，运动条件下的雌性大鼠显示出miR-1b和BDNF mRNA的显著上调（<0.001）。此外，miR-1b与认知功能（<0.05）和BDNF mRNA（<0.01）呈正相关。
结论
我们的数据表明，规律的跑步机运动可以逆转海马体miR-1b的下调，其可能在睡眠剥夺诱导的认知障碍中发挥作用。
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