AgroParisTech, Paris, France.
Department of Animal Breeding and Genetics, Swedish University of Agriculture Science, Uppsala, Sweden.
PLoS One. 2019 Jun 20;14(6):e0218719. doi: 10.1371/journal.pone.0218719. eCollection 2019.
Gastrointestinal nematodes (GIN) are a major constraint for small ruminant production. Due to the rise of anthelmintic resistance throughout the world, alternative control strategies are needed. The development of GIN resistance breeding programs is a promising strategy. However, a better understanding of the mechanisms underlying genetic resistance might lead to more effective breeding programmes. In this study, we compare transcriptome profiling of abomasal mucosa and lymph node tissues from non-infected, resistant and susceptible infected Creole goats using RNA-sequencing. A total of 24 kids, 12 susceptible and 12 GIN resistant based on the estimated breeding value, were infected twice with 10,000 L3 Haemonchus contortus. Physiological and parasitological parameters were monitored during infection. Seven weeks after the second infection, extreme kids (n = 6 resistant and 6 susceptible), chosen on the basis of the fecal egg counts (FEC), and 3 uninfected control animals were slaughtered. Susceptible kids had significantly higher FEC compared with resistant kids during the second infection with no differences in worm burden, male and female worm count or establishment rate. A higher number of differentially expressed genes (DEG) were identified in infected compared with non-infected animals in both abomasal mucosa (792 DEG) and lymph nodes (1726 DEG). There were fewer DEG in resistant versus susceptible groups (342 and 450 DEG, in abomasal mucosa and lymph nodes respectively). 'Cell cycle' and 'cell death and survival' were the main identified networks in mucosal tissue when comparing infected versus non-infected kids. Antigen processing and presentation of peptide antigen via major histocompatibility complex class I were in the top biological functions for the DEG identified in lymph nodes. The TGFβ1 gene was one of the top 5 upstream DEG in mucosal tissue. Our results are one of the fist investigating differences in the expression profile induced by GIN infection in goats.
胃肠道线虫（GIN）是小反刍动物生产的主要制约因素。由于全世界驱虫剂耐药性的上升，需要替代控制策略。开发 GIN 耐药性育种计划是一种很有前途的策略。然而，更好地了解遗传抗性的机制可能会导致更有效的育种计划。在这项研究中，我们使用 RNA 测序比较了未感染、抗性和易感感染的克里奥尔羊的胃黏膜和淋巴结组织的转录组谱。总共 24 只羔羊，根据估计的育种值，12 只是易感的，12 只是 GIN 抗性的，用 10000 个 L3 捻转血矛线虫感染两次。在感染过程中监测生理和寄生虫参数。第二次感染后 7 周，根据粪便卵计数（FEC）选择极端的羔羊（n = 6 个抗性和 6 个易感），以及 3 个未感染的对照动物被屠宰。在第二次感染期间，易感羔羊的 FEC 明显高于抗性羔羊，但在蠕虫负荷、雌雄虫计数或定植率方面没有差异。在胃黏膜（792 个 DEG）和淋巴结（1726 个 DEG）中，与未感染动物相比，感染动物中鉴定出更多的差异表达基因（DEG）。与易感组相比，抗性组的 DEG 较少（胃黏膜和淋巴结分别为 342 和 450 个 DEG）。在比较感染与未感染的羔羊时，“细胞周期”和“细胞死亡和存活”是黏膜组织中主要鉴定的网络。抗原加工和通过主要组织相容性复合体 I 呈递肽抗原是淋巴结中鉴定的 DEG 的顶级生物学功能之一。TGFβ1 基因是黏膜组织中前 5 个上调 DEG 之一。我们的研究结果是首次研究 GIN 感染在山羊中诱导的表达谱差异之一。
